NEURONAL GLIA CULTURE FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 “神经元胶质细胞培养设施”(NGF)是一个核心设施，其目的是：1)向神经科学家提供细胞或组织培养；2)培训、建议和支持该领域的新研究人员；3)为选择学习程序和自己进行培养的研究人员提供培训。NGF是一个虚拟设施，因为它不占用物理空间，它利用现有的实验室和设备。NGF人员包括一名协调员P.A.费尔奇明博士、一名研究技术员布伦达·夸德拉多夫人和一名访问顾问。这些服务包括来自产前皮质或海马区的原代神经元培养；来自新生大脑皮层的星形胶质细胞；器官型海马片；以及自去年以来的干细胞和神经球。此外，还使用免疫细胞化学方法监测提供给调查人员的培养物的质量。拟议预算包括人员薪金、咨询费和用品。顾问将根据用户对新程序的需求来选择。NGF极大地促进了这些高度专业化的制剂的使用，为研究人员留下了更多的时间进行实验工作。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The "Neuronal Glia Culture Facility" (NGF) is a core facility programmed to: 1) provide cell or tissue cultures to neuroscientists; 2) train, advice and support investigators new to this field; 3) provide training to researchers that choose to learn the procedures and do the cultures themselves. NGF is a virtual facility since it does not occupy physical space, it uses existing labs and equipment. NGF personnel consist of a coordinator, Dr. P.A. Ferchmin, a research technician, Mrs. Brenda Cuadrado, and a visiting consultant. The services include primary neuronal cultures from prenatal cortex or hippocampus; astrocytes from neonatal cerebral cortex; organotypic hippocampal slices and since last year, stem cells and neurospheres. In addition, immunocytochemical methods are used to monitor the quality of the cultures provided to the investigators. The proposed budget consists of personnel salaries, consultant fees and supplies. The consultant will be chosen according to the users needs for new procedures. NGF greatly facilitates the use of these highly specialized preparations leaving more time to the investigators for experimental work.