ORGANIZATION OF GPI ANCHORED PROTEINS IN EPITHELIAL CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 用N&B技术研究了几种GPI锚定蛋白与绿色荧光蛋白(GFP)和mCherry融合后在MDCK细胞系中的高尔基体和质膜组织。分析是在未极化和极化的池中进行的。为了收集相关数据，分别在“matek”小室和滤光片上培养细胞，并使用配备光子计数探测器的奥林巴斯倒置激光扫描共聚焦显微镜对活细胞进行图像采集。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The N & B technique has been used to study the golgi and plasmamembrane organization of several GPI anchored proteins fused to GFP (Green fluorescent protein) and mCherry, in a MDCK cell line. The analysis has been performed in unpolarized and polarized cells. In order to collect relevant data, cells have been grown respectively in "Matek" chambers and on filters, and image acquisition has been done on live cells, using an Olympus, inverted, laser scanning confocal microscope, equipped with photon counting detector.