IMPROVEMENT, UPGRADING, RESTRUCTURING OF EXISTING LFD INSTRUMENTATION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 所有现有的五个显微镜LFD设置必须进行仪器升级。鉴别器必须由常数分数鉴别器取代，所有仪器上必须安装新的前置放大器。这些修改是更换所有仪器上的检测电子设备的先决条件。 除了显微镜设置，各种基于比色皿的时间分辨荧光测量荧光计等待升级或将重新组装。所有荧光计都应通过ISS公司的新的用户友好的基于Windows的控制软件进行控制，此外还可通过软件包SimFCS进行可选控制，该软件包由我们的实验室向公众提供，用于所有显微镜设置的仪器控制和数据采集和分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. All previously existing five microscope setups of the LFD have to be subjected to instrumental upgrading. Discriminators have to be displaced by constant fraction discriminators and new pre-amplifiers have to be installed on all instruments. These modifications are the prerequisite for the replacement of the detection electronics on all instruments. In addition to the microscope setups, various cuvette-based fluorometers for time-resolved fluorescence measurements await upgrading or will be newly assembled. All fluorometers shall be controlled by the new and user-friendly Windows-based control software of the company ISS in addition to optional control via the software package SimFCS, which is made available to the general public by our laboratory and used for instrument control and data acquisition and analysis of all microscope setups.