STRUCTURAL STUDIES OF THE EUKARYOTIC TRANSCRIPTION MACHINERY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 转录是基因表达途径的第一步，也是基因表达的关键控制点。转录调控是发育、肿瘤发生和其他基本过程的基础。在真核生物中，RNA聚合酶II（pol）负责信使RNA的转录，使pol？的调控中心的基因表达。在此期间的建议，我们将继续我们的结构研究的pol转录机制。这些研究目前正在进行沿着三个途径：第一，详细分析的延伸polII使用突变分析;第二，共晶体的polII与起始和延伸因子;第三，结构研究的调解人复杂的，一个22多肽复合物所需的pol响应调节蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcription is the first step and the key control point in the pathway of gene expression. Transcriptional regulation underlies development, oncogenesis, and other fundamental processes. In eukaryotes the enzyme RNA Polymerase II (pol) is responsible for transcription of messenger RNA making pol?s regulation central to gene expression. During the period of this proposal we will continue our structural studies of the pol transcription machinery. These studies are currently proceeding along three avenues: first, detailed analysis of the elongating pol II using mutation analysis; second, co-crystals of pol II with initiation and elongation factors; third, structural studies of the mediator complex, a 22 polypeptide complex required for pol to respond to regulating proteins.