DYNAMIC STRUCTURE OF L-O AND L-D PHASES IN SPM-DOPC-CHOLESTEROL BY ESR

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 为了将SPM/DOPC/胆固醇模型脂质系统的应用扩展到真实的质膜，通过ESR谱对相边界上的Lo和Ld相进行了动态表征，以与真实的质膜进行比较。 序参量和动态参量，如旋转扩散系数，可以通过用动态模型拟合ESR谱来获得，并且这些参量可以用于表征脂质相。 如果来自模型膜相的参数表现出与从真实的质膜中的相同自旋探针获得的那些参数相似的特性，则我们可以有把握地说，模型膜与真实的膜相似。 可以采用许多不同的膜自旋探针来增加表征的特异性以及增加对相态的理解。 这种表征结合“全球”的联络线配置的Lo+Ld相共存区域将允许一个更详细的模型脂筏。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To expand the application of the SPM/DOPC/cholesterol model lipid system to real plasma membranes, the dynamic characterization of the Lo and Ld phases on the phase boundary by ESR spectra has been undertaken for comparison to real plasma membranes. The order parameter and dynamic parameters, such as rotational diffusion coefficients, can be obtained from fitting an ESR spectrum with a dynamic model, and these parameters can be used to characterize a lipid phase. If parameter from a model membrane phase exhibits similar characteristics to those parameters obtained from the same spin-probe in real plasma membranes, then we can say with confidence that the model membrane is similar to the real membrane. Many different membrane spin-probes can be employed to increase the specificity of the characterization as well as increasing the understanding of the phase state. This characterization combined with the "global" tie-line configuration for the Lo+Ld phase coexistence region will allow a more detailed model for lipid rafts.