SC COBRE: LIPIDOMICS CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 生物活性脂质的多样性及其相互关联的代谢提供了一个调节细胞内和细胞间信号和功能的通路网络。这些通路的功能障碍有助于特定疾病的病理生物学，如癌症进展和转移、加速衰老、炎症和真菌致病。这强调了发展脂质化学和分析的必要性。脂质组学核心是基于脂类化学、分析和生物学领域的关键人员的独特专业知识而创建的，现已发展成为一个机构、国家甚至国际资源，服务于鞘氨醇领域研究界的需求。 该中心提供以下方面的概念和实践培训：脂类学、不同生物材料(细胞、组织、生物流体)中的脂类成分的定性和定量分析、研究脂类代谢的合成分子工具(功能化和荧光神经酰胺、特定部位的放射性鞘磷脂)、用于细胞、体外和体内研究的各种合成脂类和类似物(神经鞘脂的细胞器靶向和鞘磷脂代谢酶的细胞器靶向抑制剂)，并协助研究人员进行实验设计、选择感兴趣的脂类和解释分析结果。分析方法基于高效液-质联用(LC-MS)技术。目前，我们提供对狮身人面像碱基及其磷酸盐、神经酰胺种类及其磷酸盐、神经鞘磷脂种类和双酰甘油种类的同时分析。葡萄糖神经酰胺处于最后发展阶段。我们的目标是达到鞘磷脂的代谢谱。核心对科布雷PIS的成功起到了重要作用，并为两个计划项目赠款和NIH颁发的竞争性共享仪器赠款获得了外部资金，这支持了我们的第二个MS仪器。核心参与了几个协作项目，并提供付费服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The diversity ofr bioactive lipids and their interconnected metabolism provides a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunction in these pathways contribute to the pathobiology of specific diseases such as cancer progression and metastasis, accelerated aging, inflammation, and fungal pathogenesis. This emphasized needs for developing lipid chemistry and analysis. The Lipidomics Core was created based on unique expertise of the key personnel in lipid chemistry, analysis and biology has evolved into an institutional, national, and even international resource that serves the needs of the research community in the field of Sphinglipids. The core provides conceptual and practical training in various aspects of lipidology, qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, biological fluids), synthetic molecular tools to study lipid metabolism (funtionalized and fluorescent ceramides, site-specific radioactive sphingolipids), diversified synthetic lipids and analogs for cellular, in vitro, and in vivo studies (organelle-targeting sphingolipids and organelle-targeting inhibitors of sphingolipid metabolizing enzymes), and assists investigators in experimental design, selection of lipid of interest and interpretation of the analytical results. Analytical approach is based on High Performance Liquid Chromatography-Tandem Mass Spectrometry (LC-MS) technology. Currently, we provide simultaneous analysis of sphingoid bases and their phosphates, ceramide species and their phosphates, sphingomyelin species and diacyl-glycerol species. Glucosylceramide is under a final developmental stage. Our goal is to reach metabolomic profile of sphingolipids. The core has been instrumental to the success of the COBRE PIs and in obtaining extramural funding for the two Program Project Grants and the award of a competitive shared instrument grant from NIH, which supported our second MS instrument. The core is engaged in several collaborative projects and provides also a paid fee-for-service.