DEVELOPMENT OF NONHUMAN PRIMATE MODELS OF ATOPIC DERMATITIS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在这个试点项目中，我们建议研究两个恒河猴模型的特应性皮炎。一个是自发发生的模型。 将检查因皮疹进入诊所的猴是否存在特征性湿疹性皮肤病变、特应性病史、皮疹的典型分布、皮肤干燥和高细菌定植。 将获得病变皮肤的皮肤活检用于组织化学分析。将获得血清以测定总IgE水平。另一种是过敏原诱导模型。我们将通过在封闭条件下，以两周为间隔，对猴进行三次为期一周的屋尘螨过敏原表皮应用，诱导猴皮炎。 在第3次表皮贴敷前后进行6 mm皮肤穿刺活检。 将在每次表皮贴敷前后采集血清。 将对活检和血清进行分析，以监测特应性皮炎的发展。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this pilot project we propose to study two rhesus monkey models of atopic dermatitis. One is a spontaneously occurring model. Monkeys admitted to the clinic for a rash will be examined for the presence of characteristic pruritic eczematous skin lesions, history of atopy, typical distribution of the rash, dry skin, and high bacteria colonization. A skin biopsy of the lesional skin will be obtained for histochemical analysis. Sera will be obtained for the total IgE levels. The other is an allergen-induced model. We will induce dermatitis in monkeys by three one-week periods of epicutaneous application of house-dust mite allergens under occlusion, at two-week intervals. 6-mm skin punch biopsies will be performed before and after the 3rd epicutaneous application. Sera will be collected before and after each epicutaneous application. Biopsies and sera will be analyzed to monitor the development of atopic dermatitis.