COMPUTATIONAL IMAGING CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这一核心设施主要为CNPRC提供光学显微镜、全幻灯片扫描、体视学、数字成像、图像数据库开发和咨询服务，但也为所有校园部门提供充值服务。其目标是帮助教职员工和学生满足他们对定性和定量成像应用的研究需求。该核心协助制作出版质量的图像，并就与其设备一起使用的实验方法提供咨询。此外，它还致力于数字化和校正来自各种来源的存档胶片图像，但主要是由CNPRC病理服务创建的那些。该核心还提供一项服务，对完全校正的数字图像进行注释并将其上传到CNPRC图像数据库。上传的图像在数据库中链接到特定动物的其他信息，并向所有获得访问权限的人提供。中国石油天然气集团公司训练有素的用户可以一周7天、每天24小时使用安装在核心中的仪器。咨询、培训和支持服务，以及其他部门的设备使用，从周一到周五8-5提供。 该设施配备了以下设备： +冰冻切片的低温恒温器 +石蜡和塑料切片的显微切割机 +塑料加工和包埋样品制备设备 +用于存档客户端、病理单元和成像核心数据的数据存储服务器(可扩展的多TB卷)。这些系统提供了对非常大的(数千兆字节)文件或数据集的快速访问，否则需要数小时才能从CNPRC的其他位置传输。 +基于Web的日历，用于仪器预订 +奥林巴斯BX61研究显微镜，配有单色和2个黑白摄像头，奥林巴斯DSU旋转盘共焦系统，计算机控制的舞台和聚焦系统，分子设备公司的变形图像捕获和分析软件，以及用于体视学和图像数据收集的智能成像创新幻灯片软件。 +第二台奥林巴斯BX61显微镜，配备由Core员工开发的用于立体技术开发的智能成像创新Slidebook软件 +图形工作站、平板扫描仪和高质量彩色校正打印机；这些设备用于出版物质量的打印、将胶片转换为数字图像、图像校正和图像存档。 +文档扫描仪，用于纸质记录的快速数字化和OCR转换 +Delta Vision显微镜系统和计算机工作站，用于极高分辨率多维荧光成像和去卷积 +一个计算机工作站，用于脱机分析立体数据(核心工作人员用于方法开发)和大型多维图像分析 +用于定量数据收集和自动图像分析的最近更新的Visiopamm VIS/CAST网格立体系统 +Adobe Photoshop、Adobe Illustrator和Adobe Lightroom软件支持Core的所有数字成像工作所有计算机都配备了分光光度色彩平衡显示器 +配备高灵敏度彩色摄像机与计算机系统连接的荧光立体显微镜 +双头Brightfield体视显微镜

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core facility provides light microscopy, whole slide scanning, stereology, digital imaging, image database development, and consultation services, primarily for the CNPRC, but also for all campus departments on a recharge basis. The goal is to assist faculty, staff, and students with their research needs for qualitative and quantitative imaging applications. This Core assists with the production of publication quality images and offers consultation on experimental approaches for use with its equipment. Additionally, it is engaged in digitizing and correcting archived film images from various sources but primarily those created by the Pathology Service at the CNPRC. The Core also provides a service to annotate and upload fully corrected digital images into the CNPRC image database. Uploaded images are linked in the database to other information for a specific animal and made available to all who have been granted access. Instrumentation housed in the Core is available 24 hours a day, 7 days a week for trained CNPRC users. Consultation, training, and support service, along with access to the equipment for other departments, is available from 8-5 Monday through Friday. The facility houses the following equipment: + A Cryostat for frozen sections + Microtomes for paraffin and plastic sectioning + Sample preparation equipment for plastic processing and embedding + Data storage servers (expandable multi-terabyte volumes) for archiving client, Pathology Unit, and Imaging Core data. These systems provide rapid access to very large (multi-gigabyte) files or data sets that would otherwise require hours to transfer from other locations in the CNPRC. + Web-based calendar for instrument reservations + An Olympus BX61 research microscope with 1 color and 2 B/W cameras, Olympus DSU spinning disk confocal system, computer controlled stage and focusing system, Molecular Devices MetaMorph image capture and analysis software, and Intelligent Imaging Innovations SlideBook software for stereology and image data collection. + A second Olympus BX61 microscope with Intelligent Imaging Innovations SlideBook software for stereology technique development by the Core's staff + A graphics workstation, flatbed scanner, and a high-quality color corrected printer; these are used for publication quality prints, converting film to digital images, image correction, and image archiving. + Document scanners for rapid digitization and OCR conversion of paper records + A Delta Vision Microscopy System and computer workstation for very high resolution multi-dimensional fluorescence imaging with deconvolution + A computer workstation for offline analysis of stereology data (used for method development by the Core staff), and large multidimensional image analysis + A recently updated Visiopharm VIS/CAST Grid Stereology System for quantitative data collection and automated image analysis + Adobe Photoshop, Adobe Illustrator, and Adobe LightRoom software to support all digital imaging efforts of the Core  all computers are outfitted with spectrophotometrically color balanced monitors + A fluorescence stereomicroscope outfitted with a high sensitivity color camera coupled to a computer system + A dual-head brightfield stereomicroscope