Malaria Vaccine: CSP-rEPA

设计、合成了编码恶性疟原虫环孢子子（CS）蛋白的几个基因，并将其克隆到大肠杆菌和帕斯德菌表达载体中。制备了一个序列与恶性疟原虫印度株对应的重组CS蛋白（rCSP）。该蛋白将与载体蛋白偶联，例如，来自aruginosa假单胞菌的重组外蛋白a，并偶联Pfs25。继续努力在毕赤酵母表达系统中产生CSP。

Several genes encoding the Plasmodium falciparum Circumsporozoite (CS) protein has been designed, synthesized, and cloned into an E. coli and P. pastoris expression vectors. One recombinant CS protein (rCSP) with sequence corresponding to P. falciparum strain India was produced. The protein will be conjugated to carrier proteins, e.g. a recombinant exoprotein A from Pseudomonas aruginosa, and conjugated Pfs25. Efforts continue to produce the CSP in the Pichia pastoris expression system. A mouse model using a transgenic berghei parasite expressing falciparum CSP on its sporozoite surface is being developed and tested as an assay to evaluate protection imparted by a CSP-based vaccine. We are also creating a transgenic knowelsi parasite, expressing falciparum CSP on its sporozoite surface, to establish a monkey challenge model for evaluating protective efficacy of CSP-based vaccines.