Superior cell culture device for more efficient biological production

描述（由申请人提供）：对于生物技术核心的许多中小型细胞培养工作，传统的培养器皿（如烧瓶和滚瓶）是唯一实用的设备。虽然它们易于使用和一次性使用，但它们的设计限制导致它们浪费劳动力，实验室空间，处理空间，生长因子，蛋白质回收材料和时间。本申请提出了一种远上级传统培养器皿的新型细胞培养装置。新设备被称为“6X膜辊瓶（6X MRB）。“它的外部形状是一个滚瓶的形状，这样它就可以与标准的实验室设备连接。然而，它具有独特的内部几何形状，使其能够在更小的空间内生长更多的细胞，浓缩细胞分泌的蛋白质，并减少生长因子的使用。预计每年可节省数百万美元。虽然设计概念在纸面上看起来很强大，但可行性需要通过生物性能测试来证明。因此，第1阶段的主要目标是确定当面临实际生物学评价时，6倍MRB概念是否会如假设那样发挥作用。具体目标如下。目标1：根据我们的概念设计制造6X MRB测试设备。相对于传统的培养器皿，表面积将增加六倍，透析膜将允许以高密度培养细胞。 可拆卸：无菌6X MRB试验器械可用于Aim 2试验。目标二：通过使用分泌EPO的BHK细胞、使用昂贵生长因子的间充质细胞和分泌病毒载体的PG13细胞进行生物学评估，确定6X MRB的可行性，以确定其相对于传统培养皿降低细胞培养过程成本的能力。 可验证：报告是否需要第II阶段。 如有必要，将提供设计数据库以指导第二阶段计划。 公共卫生相关性：对于生物技术核心的许多中小型细胞培养工作，传统的培养器皿如烧瓶和滚瓶是唯一实用的设备。虽然它们易于使用和一次性使用，但它们浪费了劳动力、实验室空间、处置空间、生长因子、蛋白质回收材料和时间。在没有上级装置替代品的情况下，浪费了花费在细胞培养上的大量资金中的很大一部分。提出了一种新的细胞培养装置，该装置克服了这些问题，并且预计每年将允许数百万美元从平凡的细胞培养活动重新定向到更重要的研究方面。

DESCRIPTION (provided by applicant): For much of the small and mid-scale cell culture work at the core of biotechnology, traditional culture ware such as flasks and roller bottles are the only devices that are practical for use. Although they are easy to use and disposable, they have design limits that cause them to squander labor, laboratory space, disposal space, growth factors, protein recovery materials, and time. This application proposes a new cell culture device that is far superior to traditional culture ware. The new device is called the "6X Membrane Roller Bottle (6X MRB)." It has an outside shape that takes the shape of a roller bottle so that it can interface with standard lab equipment. However, it has a unique internal geometry that allows it to grow more cells in less space, concentrate cell secreted protein, and reduce the use of growth factors. Millions of dollars of savings are projected annually. While the design concept appears strong on paper, feasibility needs to be proven through biological performance testing. Thus, the primary goal of Phase 1 is to determine if the 6X MRB concept will perform as hypothesized when faced with actual biological evaluations. Specific aims follow. Aim 1: Fabricate 6X MRB test devices in accordance with our concept design. A six fold increase in surface area will be present relative to traditional culture ware and a dialysis membrane will allow cells to be cultured at high density. Deliverable: Sterile 6X MRB test devices ready for Aim 2 testing. Aim 2: Determine feasibility of the 6X MRB by conducting a biological assessments with BHK cells secreting EPO, mesenchymal cells using expensive growth factors, and PG13 cells secreting viral vectors to determine its capacity to cost reduce the cell culture process relative to traditional culture ware. Deliverable: Report whether or not Phase II is warranted. If warranted, a design data base will be available to guide the Phase II plan. PUBLIC HEALTH RELEVANCE: For much of the small and mid-scale cell culture work at the core of biotechnology, traditional culture ware such as flasks and roller bottles are the only devices that are practical for use. Although they are easy to use and disposable, they squander labor, laboratory space, disposal space, growth factors, protein recovery materials, and time. In the absence of a superior device alternative, a significant portion of the vast amount of money spent on cell culture is wasted. A new cell culture device is proposed that overcomes these problems and is expected to allow millions of dollars annually to be redirected from mundane cell culture activity to much more important aspects of research.