A Walk-UP LC-MS for the Rapid Analysis of Organic Compounds

描述（申请人提供）：。此共享仪器拨款申请要求使用液相色谱-质谱计（LC-MS）。该仪器由一个高效液相色谱系统和一个正交飞行时间ESI/APCI质谱仪组成，并将作为直接“开放获取”的仪器设置给研究人员（研究生、博士后、本科生和研究技术人员）。对复杂分子样品进行化学表征的能力是各个学科的核心，尤其是化学科学。该仪器的用户将主要由从事对生物学和人类医学具有重要意义的新型有机和有机金属分子合成的研究人员组成。配备自动进样器的多功能LC-MS仪器和学生操作的“行走”访问软件将显著推进几个令人兴奋的美国国立卫生研究院资助的研究项目。这些努力都依赖于在每个合成过程中形成的分子产物的快速结构解析，因此直接依赖于研究人员立即可用的分子分析技术。开放获取的LC-MS仪器的可用性允许在进行反应时立即访问反应的质量剖面数据，不仅可以提高反应的执行速度，还可以提高每个反应可以科学评估的准确性。这将大大加快在制备具有生物学重要性的分子方面取得新发现的速度。美国国立卫生研究院资助的相关项目包括但不限于开发新的合成方法，用于制备具有生物和治疗意义的天然和非天然分子。这些目标包括以靶标和多样性为导向的新型抗菌、抗病毒和抗肿瘤药物的合成，各种天然和非天然产物，类固醇受体的分子探针，乳腺肿瘤成像的放射性药物，以及生物医学传感器阵列的小分子蛋白质模拟物。

DESCRIPTION (provided by applicant): . A Liquid Chromatography-Mass Spectrometer (LC-MS) is requested for this Shared Instrumentation Grant application. The instrument consists of a High Performance Liquid Chromatography system coupled to an orthogonal time-of-flight ESI/APCI mass spectrometer, and is to be set up as direct "open-access" instrumentation to researchers (graduate students, postdoctoral fellows, undergraduates, and research technicians). The ability to chemically characterize complex molecule samples lies at the heart of various disciplines, including the chemical sciences in particular. The users of this instrumentation will primarily consist of researchers engaged in the synthesis of novel organic and organometallic molecules that are of importance to biology and human medicine. A versatile LC-MS instrument equipped with an autosampler and "walk-on" access software for student operation will significantly advance several exciting NIH-funded research projects. Each of these efforts depends on the rapid structure elucidation of the molecular products formed in each synthetic process and is therefore directly dependent on the molecular analysis techniques immediately available to the researcher. The availability of open-access LC-MS instrumentation, which allows for immediate access to mass profile data of reactions as they are being performed, will not only increase the rate at which reactions can be performed, but also enhance the accuracy with which each reaction can be scientifically evaluated. This would greatly accelerate the rate at which novel discoveries can be made in the preparation of molecules of biological importance. The associated NIH-funded projects include, but are not limited to, the development of new synthetic methods for the preparation of natural and non-natural molecules of biological and therapeutical interest. These goals involve both target- and diversity-oriented synthesis of novel anti-bacterial, antiviral and antitumor agents, various natural and non-natural products, molecular probes for steroid receptors, radiopharmaceuticals for breast tumor imaging, and small molecule protein mimics for biomedical sensor arrays. PUBLIC HEALTH RELEVANCE: Several NIH-funded investigators in the Department of Chemistry at the University of Illinois seek funding to purchase an instrument for combined liquid chromatography/mass spectrometry. Such an instrument will significantly enhance our ability to analyze organic reactions, ultimately leading to much more rapid progress toward our project goals.