Roche 454 Next Generation Sequencer for Human Microbiome and Infectious Disease R

描述(由申请人提供)： 摘要纽约大学朗格尼医学中心Skirball研究所内的DNA测序设备为纽约大学社区的大约200个实验室和数百名科学家提供测序服务。作为核心设施，测序实验室提供最先进的设备和专业知识，这对大多数单独的实验室来说是不切实际的。我们正在申请购买罗氏基因组测序仪FLX(GS FLX)的资金，该设备由罗氏公司旗下的454测序公司开发。这种焦磷酸测序仪在10小时的仪器运行时间内产生了100多万个独特的高质量测序读数，平均长度为350到400个碱基对。每次仪器运行的总平均产量为400至500兆数据库，由于可以在一周内多次运行仪器，因此可以产生数十亿数据库的可用数据。该仪器支持多种应用，包括从头病原体测序、元基因组学、靶区重测序、转录组测序、SNP鉴定、小RNA分析、拷贝数变异和DNA甲基化。该平台将为医疗中心提供支持正在进行的NIH资助项目的能力，与传统的Sanger技术相比，成本显著降低，周转时间可以用几天到几周来衡量，而不是几个月到几年。这样的实验将大大提高我们对牛皮癣、胃食道反流病等皮肤、肠道和胃食道疾病以及疟疾和艾滋病毒等传染病的病因和机制的了解。

DESCRIPTION (provided by applicant): ABSTRACT The DNA Sequencing Facility within the Skirball Institute at New York University Langone Medical Center provides sequencing services to about 200 labs and hundreds of scientists in the NYU community. As a core facility, the sequencing lab offers state-of-the-art equipment and expertise, which would be impractical for most individual labs to maintain. We are requesting funds to purchase a Roche Genome Sequencer FLX (GS FLX), developed by 454 Sequencing, a Roche corporation. This pyrosequencing instrument generates over one million unique high quality sequencing reads with an average of 350 to 400 bases pairs in length within a ten hour instrument run time. The total average yield per instrument run is 400 to 500 megabases, and since it is possible to run the instrument multiple times a week, gigabases of useable data can be produced. The instrument supports a wide variety of applications, including de novo pathogen sequencing, metagenomics, target region resequencing, transcriptome sequencing, SNP identification, small RNA analysis, copy number variation, and DNA methylation. The platform would provide the Medical Center with the ability to support ongoing NIH-funded projects at a significantly reduced cost compared to traditional Sanger technologies, with a turn around time that can be measured in days to weeks instead of months to years. Such experiments will greatly increase our understanding of the causes and mechanisms of a number of diseases of the skin, intestinal tract and gastroesophageal tract, such as psoriasis and gastroesophageal reflux disorders, as well as infectious diseases such as malaria and HIV.