NanoString Instrumentation

描述（由申请人提供）：Whitehead研究所申请23.5万美元用于购买NanoString Technologies公司的nCounter系统。nCounter系统是一种新的基因表达分析方法，具有更高的复用率、更高的通量和更低的成本，提供了实时PCR的数据质量。“nCounter”系统将填补高通量基因表达项目的空白，该项目需要在数百到数千个样本中进行中程多路复用（50-500个基因）。传统的基因表达技术如实时荧光定量PCR或微阵列无法很好地满足这一通量范围。该系统独特的检测技术也激发了传统RT-PCR和微阵列技术不足的新应用建议。“nCounter”系统将被整合到怀特黑德研究所基因组技术核心（GTC）的技术组合中。GTC为怀特黑德研究所的20个实验室和世界各地的几十个实验室提供服务，并且作为一个收费的基因组核心设施，它在成功支持共享仪器方面有着悠久的历史。nCounter系统将补充现有的基于微阵列和定量PCR的基因表达分析平台，为各种规模的表达分析项目提供完整的解决方案。

DESCRIPTION (provided by applicant): The Whitehead Institute requests $235,000 for the purchase of an nCounter" system from NanoString Technologies. The nCounter" system is a new approach to gene expression analysis that offers the data quality of Real-Time PCR with higher multiplexing, better throughput and lower cost. The nCounter" system will fill a niche for high throughput gene expression projects requiring mid-range multiplexing (50-500 hundred genes) in hundreds up to thousands of samples. This throughput range is not well served by traditional gene expression technologies like Real-Time PCR or microarrays. The system's unique detection technology also inspires proposals for novel applications where traditional technologies of RT-PCR and microarrays are inadequate. The nCounter" system will be integrated into the technology portfolio of the Whitehead Institute's Genome Technology Core (GTC). The GTC serves 20 labs at the Whitehead Institute and dozens more around the world and has a long history of successfully supporting shared instrumentation as a fee-for-service genome core facility. The nCounter system will complement existing gene expression analysis platforms based on microarrays and quantitative PCR to provide complete solutions for expression analysis projects of all sizes. PROJECT NARRATIVE: The ability to measure levels of gene expression in cells is a critical component of nearly every biomedical research project today. Traditional technologies for gene expression analysis are expensive, time consuming and suffer from a variety of technical limitations. The nCounter(tm) from NanoString is a revolutionary technology that will enable many research projects by providing a cheaper, faster and technically superior option for high throughput expression analysis.