JEOL JEM-1400 Transmission Electron Microscope with AMT-XR611M Digital Camera Sys

描述（由申请人提供）：本申请的广泛、长期目标是获得资金，为堪萨斯大学医学中心（KUMC）的电子显微镜研究实验室（EMRL）购买一台新的JEOL JEM-1400透射电子显微镜（TEM）。该核心设施自1972年以来一直在运营，并不断获得KUMC的年度财政支持。EMRL拥有一流的，经验丰富的技术人员，并包含所有必要的辅助设备，使用TEM和扫描电子显微镜（SEM）进行广泛的实验。然而，唯一可用的TEM手术仪器是超过24年的JEOL JEM-100CX II。这种显微镜现在已经过时了。几个NIH资助的研究各种健康相关主题的团队目前依赖于KUMC的EMRL。这些项目包括探讨以下机制的项目：（a）正常肾小球毛细血管发育和缺陷导致滤过屏障特性丧失;（B）神经元突触的形成;（c）肠蠕动障碍和囊性纤维化中细菌过度生长;（d）氧化应激对衰老肌肉和大脑造成的损害;（e）体细胞-性腺生殖细胞相互作用，这对生育力至关重要;（f）对细胞分裂重要的细菌细胞骨架的组成和功能;（g）灵长类动物模型中HIV-AIDS的发病机理;和（f）选择的脂肪酸对人乳腺癌细胞的抗癌活性。所有这些项目的进展将随着可靠的现代TEM的可用性而得到加强，并且通过使用所要求的AMT数字相机系统和5个样品网格保持器也将增加吞吐量。

DESCRIPTION (provided by applicant): The broad, long term objective of this application is to obtain funds to purchase a new JEOL JEM-1400 transmission electron microscope (TEM) for the Electron Microscopy Research Laboratory (EMRL) at the University of Kansas Medical Center (KUMC). This core facility has been in operation since 1972, and has received annual financial support from KUMC continuously. The EMRL has superb, experienced technical staff and contains all of the necessary ancillary equipment to carry out a wide range of experiments using TEM and scanning electron microscopy (SEM). However, the only operative TEM instrument available is a JEOL JEM-100CX II that is more than 24 years old. This microscope is now obsolete. Several NIH-funded teams researching a diverse set of health-related topics currently rely on the EMRL at KUMC. These include projects that address mechanisms of (a) normal kidney glomerular capillary development and defects leading to loss of filtration barrier properties; (b) formation of neuronal synapses; (c) intestinal dysmotility and bacterial overgrowth in cystic fibrosis; (d) damage induced by oxidative stress in aging muscle and brain; (e) somatic cell-gonadal germ cell interactions critical for fertility; (f) composition and functions of the bacterial cytoskeleton important for cell division; (g) pathogenesis of HIV-AIDS in a primate model; and (f) anti-cancer activity of select fatty acids on human breast cancer cells. Progress on all of these projects will be enhanced with the availability of a reliable, modern TEM, and throughput will also be increased with access to the requested AMT digital camera system and 5 sample grid holder.