Multi-Metal Chip for Point-of-Care Monitoring in Children

描述(申请人提供)：本申请旨在开发一种“多金属芯片”，便于快速准确地测量儿童血液中的金属浓度，包括锰(锰)、锌(锌)、镉(镉)和铅(铅)。全血分析是确定金属暴露和评估健康结果的最常见方法，但从儿童身上采集样本面临多重挑战，包括父母同意和子女同意，以及献血能力(特别是在需要重复测量的研究中)。这项应用旨在通过展示一种“芯片实验室”传感器来改变目前的模式，该传感器能够对少量血液样本(一次柳叶刀穿刺法两滴)进行快速分析(5-10分钟)。我们建议开发这一用于高灵敏度测量全血中锰、锌、镉和铅的系统，以实现以下具体目标：1)开发一种芯片上实验室传感器，用于快速、即时地进行全血中多金属分析；以及2)进行现场测试，并确定该多金属传感器在测量居住在锰铁精炼厂附近儿童的血液金属浓度方面的适用性。我们的方法将是将阳极溶出伏安法与我们在芯片实验室和微流控方面的深厚专业知识相结合。我们的工程师、化学家和环境健康多学科团队已经迈出了开发这种设备的第一步。我们已经通过试点测试证明了传感器的可行性，现在准备全面开发用于护理点应用的传感器系统。这项研究的独特之处在于对儿童友好的要求，即只需滴血进行分析，成本低(一次性)，能够进行多次血液测量，并可能用于大型临床、职业和研究环境，如国家儿童研究和国家健康和营养检查调查。 与公共健康相关：这项应用寻求开发一种“芯片实验室”传感器，用于快速准确地测量血液中的金属(锰、锌、镉和铅)。全血分析是确定金属暴露和评估健康结果的最常见方法，但从儿童身上采集样本是非常有问题的。这项研究的独特之处在于对儿童友好的要求，只需一滴血进行分析，成本低，能够进行多次血液测量，并有可能在大规模的临床、职业和研究环境中使用。

DESCRIPTION (provided by applicant): This application seeks to develop a "multi-metal chip" that will facilitate rapid and accurate point- of-care measurement of blood metal concentration, including manganese (Mn), zinc (Zn), cadmium (Cd), and lead (Pb) in children. Whole blood analysis is the most common method for determining metal exposure and assessment of health outcomes, yet sample collection from children poses multiple challenges, including parent consent and child assent, and the ability to give blood (especially in studies that require repeated measurements). This application aims to transform the current paradigm by demonstrating a "laboratory-in-a-chip" sensor capable of rapid analysis (5-10min) on small blood samples (2 drops from a single lancet puncture). We propose to develop this system for highly sensitive measurement of Mn, Zn, Cd, and Pb in whole blood by accomplishing the following specific aims: 1) develop a lab-on-a-chip sensor for a rapid, point-of-care multi-metal analysis in whole blood, and 2) conduct a field test and determine the applicability of the multi-metal sensor for measuring blood metal concentration in children residing near a ferromanganese refinery. Our approach will be to integrate anodic stripping voltammetry with our deep expertise in lab-on-a-chip and microfluidics. Our multidisciplinary team of engineers, chemists, and environmental health has taken the first steps towards developing such a device. We have demonstrated feasibility of the sensor through pilot tests, and are now poised to fully develop the sensor system for point-of-care application. The uniqueness of this study is the child-friendly requirement of only drops of blood for analysis, low- cost (disposable), ability to conduct multiple blood measurements, and its potential use in large- scale clinic, occupational and research settings, such as the National Children's Study and the National Health and Nutrition Examination Survey. PUBLIC HEALTH RELEVANCE: This application seeks to develop a "laboratory-in-a-chip" sensor for rapid and accurate point-of- care measurement of metals (manganese, zinc, cadmium, and lead) blood. Whole blood analysis is the most common method for determining metal exposure and assessment of health outcomes, yet sample collection from children is highly problematic. The uniqueness of this study is the child-friendly requirement of only drops of blood for analysis, low-cost, ability to conduct multiple blood measurements, and potential use in large-scale clinic, occupational and research settings.