PROMOTER-BASED FUNCTIONAL MAPPING OF AMYGDALA MICROCIRCUITS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在报告期间，我们完成了我们的调查胆囊收缩素（CCK）含有中间神经元使用CCK启动子标记的绿色荧光蛋白（GFP）的生理和形态学特性。 然后将所得慢病毒构建体用于体内转染神经元，仅那些正常表达CCK的神经元共表达GFP。 我们已经用这种方法来研究大鼠基底外侧杏仁核CCK中间神经元的生理特性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the reporting period we completed our investigation of the physiological and morphological properties of cholecystokinin (CCK) containing interneurons using the CCK promoter tagged with a green fluorescent protein (GFP). The resulting lentiviral construct was then used to transfect neurons in vivo, only those neurons that normally express CCK co-expressed GFP. We have used this approach to examine the physiological properties of CCK interneurons in the rat basolateral amygdala.