GENETIC ANALYSIS OF GERM CELL FORMATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项研究的目的是测量移植到非人灵长类动物睾丸中的ES细胞的生殖细胞发育及其在生精小管内完成减数分裂的能力。具体地说，我们将首先进行一系列模型开发研究，旨在确定通过植入睾酮来下调猴子精子发生的可行性，以及通过口服双硫丹来消除精原干细胞群的可行性。接下来，左侧的睾丸将接受尚未进行体外分化的人类ES细胞群体，而右侧的睾丸将接受暴露于14天分化的细胞。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this study is to measure germ cell development of the ES cells and their ability to complete meiosis within the seminiferous tubules after transplantation into nonhuman primate testis. Specifically we will first conduct a series of model development studies designed to establish the feasibility of down-regulating spermatogenesis in monkeys with testosterone implants and eliminating spermatogonial stem cell populations with orally administered bisulfan exposure. Next, the left testis will receive populations of human ES cells that have not been subjected to in vitro differentiation while the right testis will receive cells exposed to 14 days of differentiation.