GENE-TARGETTED SNP DISCOVERY IN RHESUS MACAQUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目旨在通过对20只无关的中国和印度恒河猴和4只食蟹猴的3' UTR进行测序，在10，000个基因区域中鉴定单核苷酸多态性（SNP）。将分析汇编的SNP数据，以确定印度和中国人群中的等位基因频率，并确定食蟹猴的共享和新等位基因。结果将在公共数据库dbSNP和MonkeySNP中报告。在过去的一年中，我们调整了SNP发现策略，以包括新的下一代测序技术。 因此，我们正在开发一个分析管道，以支持由Illumina GenomeAnalyzer生成的外显子捕获的基因组序列的研究。迄今为止发现的所有SNP都提交给dbSNP和monkeySNP供公众访问。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project aims to identify single nucleotide polymorphisms (SNPs) in 10,000 gene regions, by sequencing the 3' UTR of 20 unrelated Chinese and Indian rhesus macaques and 4 cynomolgus macaques. The compiled SNP data will be analyzed to identify allele frequencies in both Indian and Chinese populations and to identify shared and novel alleles with cynomolgus macaques. The results will be reported in the public databases dbSNP and MonkeySNP. In the past year we adjusted our SNP discovery strategy to include new next gen sequencing technologies. Thus we are developing an analysis pipeline to support the study of exon-captured genomic sequence, generated by an Illumina GenomeAnalyzer, All SNPs discovered to date were submitted to dbSNP and monkeySNP for public access.