ANALYTICAL CORE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 分析核心实验室 COCVD的分析核心为参与研究人员提供技术服务和支持，用于检测和定量与肥胖和心血管疾病的诊断，治疗和实验研究相关的生物分子。核心的一个主要目的是管理和操作复杂的先进仪器，这将超出个人参与调查的能力。 这包括一个BioPhase溶液阵列酶标仪，气相色谱仪和复杂和先进的仪器，用于小分子分析和串联质谱法的结构表征。 后一种仪器，形成了核心最独特能力的主要焦点，包括两个混合三重四重线性离子阱质谱仪，具有一系列与自动HPLC系统接口的离子源。 这些仪器之一可以与基质辅助激光解吸离子源一起操作。 另一个可以用自动纳米电喷雾电离源操作。 这些配置使我们能够分析和定量各种各样的分子，并进行公正的代谢物分析研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Analytical Core Laboratory The analytical core of the COCVD provides technical services and support to participating investigators in the broad area of detection and quantitation of biological molecules of relevance to the diagnosis, treatment and experimental investigation of obesity and cardiovascular diseases. A major purpose of the core is to administer and operate sophisticated advanced instrumentation that would be beyond the capabilities of individual participating investigators. This includes a BioPlex solution array microplate reader, a gas chromatograph and sophisticated and advanced instrumentation for small molecule analysis and structural characterization by tandem mass spectrometry. This latter instrumentation, which forms the primary focus of the most unique capabilities of the core, includes two hybrid triple quadruple linear ion trap mass spectrometers with a range of ion sources that are interfaced to automated HPLC systems. One of these instruments can be operated with a matrix assisted laser desorption ion source. The other can be operated with an automated nano electrospray ionization source. These configurations allow us to analyze and quantitate a wide variety of molecules and to also undertake unbiased metabolite profiling studies.