CENTER FOR THE ANALYSIS OF CELLULAR MECHANISMS AND SYSTEMS BIOLOGY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这个项目的目标是发展一个多学科的细胞机制和系统生物学分析中心，提供新的基础设施来促进对细胞机制的理解，更好地了解如何克服疾病，并在这一领域建立一支关键的教师队伍。该中心在蒙大拿州和周围的五个州是独一无二的，并将包括分析细胞机制的能力组合。该项目将创建一个强大的质谱学和蛋白质组学核心基础设施，旨在培训研究人员。大多数细胞机制似乎是由蛋白质翻译后修饰控制的，MS和蛋白质组学核心将增加新的质谱仪来改进蛋白质修饰的表征，高灵敏度微流控纳米LC/MS/MS，增强多色蛋白质组多路传输到随生物刺激改变的点状蛋白质的高光谱成像仪，以及用于高效回收针状点状蛋白质的蛋白质电洗脱和微流控消化系统。核心将包括密歇根州立大学开发的灵敏差异蛋白质检测能力和高灵敏微流控纳米LC/MS/MS。新的软件将整合来自现有DNA微阵列设施的数据。蛋白质组学试剂合成核心将制备新的荧光试剂，用于差异分析生物刺激引起的酶活性变化和翻译后修饰。 将通过支持三名初级教员和一名高级教员的发展来建立临界质量，后者正在制定他的研究的新路线。优秀的地方和国家导师和外部咨询委员会成员将指导这些教师和中心的发展。在项目期间，我们计划在需要建立临界质量的领域招聘四名新教师：一名化学生物学家/有机合成专家，一名使用先进MS工具的生物质谱学家，一名从事代谢建模和代谢组学工作的系统生物学家，以及一名使用全球分子工具的细胞生物学家。杰出的密歇根州立大学机构支持、细胞机制的后基因组分析的重要性和及时性，以及对先进方法培训的导向，将催生新的项目--并将产生重大的科学贡献和一个可持续的中心。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to develop a multidisciplinary Center for the Analysis of Cellular Mechanisms and Systems Biology, to provide new infrastructure to advance understanding of cellular mechanisms, to better understand how to overcome disease, and to build a critical mass of faculty in this area. The Center is unique in Montana and the five surrounding states, and will include a combination of capabilities for analysis of cellular mechanisms. This project will create a powerful Mass Spectrometry and Proteomics Core infrastructure, oriented toward training researchers. Most cellular mechanisms appear to be controlled by protein post-translational modifications and the MS and Proteomics Core will add new mass spectrometers for improved characterization of modifications of proteins, high sensitivity microfluidic nano LC/MS/MS, a Hyperspectral imager for enhanced multicolor proteomic multiplexing to pin-point proteins that change with biological stimulation, and a protein electro-elution and microfluidic digestion system for high efficiency recovery of pin-pointed proteins. Sensitive differential protein detection capabilities developed at MSU and high sensitivity microfluidic nano LC/MS/MS will be included in the Core. New software will integrate data from an existing DNA microarray Facility. A Proteomics Reagent Synthesis Core will prepare new fluorescent reagents for differential analysis of changes in enzyme activity and post-translational modifications that result from biological stimulation. Critical mass will be built by supporting the development of three junior faculty and one senior faculty member, who is charting a new course in his research. Outstanding local and national mentors and External Advisory Committee members will guide the development of these faculty and the Center. Over the period of the project we plan to hire four new faculty in areas needed to build critical mass: a Chemical Biologist/Organic Synthesis expert, a Biological Mass Spectroscopist using advanced MS tools, a Systems Biologist working on metabolic modeling and metabolomics, and a Cell Biologist using global molecular tools. Outstanding MSU Institutional Support, the significance and timeliness of the post-genomic analysis of cellular mechanisms, and the orientation toward training in advanced methods will seed new projects-and will result in significant scientific contributions and a sustainable Center.