COBRE: UVM MED: CORE C: CELLULAR & MOLECULAR

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 佛蒙特州大学神经科学科布雷细胞和分子生物学核心设施为希望在研究中利用细胞和分子生物学但可能没有必要的经验或设备的研究人员提供设备和专业知识。该设施包括以下主要设备：赛弗吉SELDI-TOF质谱仪、蔡司掌上激光显微解剖系统、2台应用生物系统7500FAST定量聚合酶链式反应系统、奥德赛红外成像仪、Qiacube自动样品制备系统、Qiagility液体处理系统、BioRad Experion自动电泳系统、BioRad书法家阵列打印机、Neuroleucida神经解剖分析系统、BioRad二维凝胶系统和Beckman超离心机。该设施还配备了标准的分子生物学实验室设备，包括三台Techne热循环仪、一台BioRad梯度循环仪、一台纳米滴分光光度计、一个发光计和细胞培养罩，以及各种孵化器和显微镜：蔡司荧光显微镜、低速离心机、摇动孵化器和其他几个孵化器。该设备被各种项目和研究人员使用，在报告所述期间，用户群包括39名教职员工、21名工作人员、8名博士后助理、29名研究生和28名本科生。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The University of Vermont Neuroscience COBRE Cell and Molecular Biology Core facility provides equipment and expertise for researchers who wish to utilize cell and molecular biology in their research but may not have the necessary experience or equipment. The facility contains the following major pieces of equipment: Ciphergen SELDI-TOF Mass Spectrometer, Zeiss Palm Laser Microdissection System, 2 Applied Biosystems 7500FAST Quantitative PCR Systems, Odyssey Infrared Imager, Qiacube Automated Sample Preparation System, Qiagility Liquid Handling System, BioRad Experion Automated Electrophoresis System, BioRad Calligrapher Array Printer, Neuroleucida Neuroanatomical Analysis System, BioRad 2-D Gel System and Beckman Ultracentrifuges. The facility is also fully equipped with standard molecular biology laboratory equipment including three Techne thermal cyclers, one BioRad gradient cycler, one Nanodrop spectrophotometer, a luminometer and cell culture hood, as well an as assortment of incubators and microscopes: a Zeiss fluorescence microscope, low-speed centrifuges, a shaking incubator, and several other incubators. This equipment is employed by a wide variety of projects and researchers, with a user base during the reporting period of 39 faculty, 21 staff members, 8 postdoctoral associates, 29 graduate students and 28 undergraduate students.