Imaging Core

自2004年以来，Imaging Core一直支持NYMC的氧化还原信号和血管生物学项目， 提高了产量和能力，以满足日益增长的研究需求。成像核心的潜力在于 基于其领导者、顾问和其他调查人员的科学专业知识， 在细胞和分子成像领域的记录。将成像技术整合到生理 研究允许实验范式产生重要的新概念， 提供了重要的见解，在疾病模型中使用的病理生理机制， 部门在上一个资助期内，我们已经出版或即将出版几份同行评审的 论文，使用成像核心设施为3个项目提供的技术和方法（见第 G）。成像核心设施设备齐全，目前有一名技术员。学院全面 承诺在有需要时扩大核心基金的范围。成像核心可以访问高度 这些精密仪器共同构成了一套独特的成像设备， 从而获得适于分析活血管中ROS信号传导的分辨率。所有服务都将 可用于小鼠、大鼠、牛、犬和人体组织以及基于细胞的研究。特别是 Imaging Core将： 1.提供各种服务，以协助测量ROS（包括Oi”和FL“）的产生， 心血管组织，和 2.提供专业知识，设施和培训的个人在执行免疫细胞化学， 血管组织的免疫组织化学和组织学（包括显微镜使用和计算机辅助 图像分析和建立协议）。核心还将负责数据共享， 数据存储和质量控制服务。它还为研究员和学生提供显微镜培训 技术. 3.为使用LCM分析特定血管细胞类型中的基因表达提供支持。 成像核心将促进所有三个项目的图像和监测活性氧的生产， 心血管组织，以检测和定位血管细胞中的蛋白质表达和3-硝基酪氨酸含量， 显微解剖内皮和平滑肌细胞以及心肌细胞用于差异基因表达分析。

The Imaging Core has supported redox signaling and vascular biology projects at NYMC since 2004 and has increased its output and capabilities to meet growing research demands. The potential of the Imaging Core is based on the scientific expertise of its leader, consultants and of the other investigators, who have strong track records in the area of cellular and molecular imaging. The integration of imaging techniques into physiological studies has allowed for experimental paradigms that have generated important new concepts as well as providing significant insights into the pathophysiological mechanisms in disease models used in the Department. Over the previous funding period, we have published or are about to publish several peer-reviewed papers, using the technologies and methods provided by the Imaging Core facility for the 3 projects (see section G). The Imaging Core Facility is fully equipped and currently staffed by one technician. The College is fully committed to expanding the scope of the Core Facility as need arises. The Imaging Core has access to highly sophisticated instruments, which taken together, constitute a unique set of imaging apparatus in terms of achieving a resolution power suitable for the analysis of ROS signaling in living blood vessels. All services will be available for mouse, rat, bovine, dog and human tissues as well as for cell-based studies. Specifically the Imaging Core will: 1. offer a variety of services to assist with measurements of ROS (including Oi" and FL^) production in cardiovascular tissues, and 2. provide expertise, facilities and training for individuals in performing immunocytochemistry, immunohistochemistry, and histology of vascular tissues (including microscope usage and computerassisted image analyses, and establishing protocols). The Core will also be responsible for data sharing, data storage and quality control services. It also provides training for fellows and students in microscopy techniques. 3. provide support for analysis of gene expression in specific vascular cell types using LCM. The Imaging Core will facilitate all three of the Projects to image and monitor ROS production in cardiovascular tissues, to detect and localize protein expression and 3-nitrotyrosine content in vascular cells and microdissect endothelial and smooth muscle cells and cardiomyocytes for differential gene expression analysis.