BIOMARKERS ANALYSIS OF PREGNANT RHESUS MONKEYS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本提案的目的是获得妊娠恒河猴的对照数据，并采集血液和粪便样本进行分析。 本研究将为之前在Yerkes进行的研究提供阴性对照组，该研究涉及用L.单核细胞增多症。在本提案中，将给予妊娠猴无菌鲜奶油（溶媒），并在不同时间间隔采集血液和粪便样本，以确定是否存在李斯特菌感染，同时从血液单核淋巴细胞中采集免疫应答参数。在最后一次血样采集后，将母鼠和后代送回殖民地。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objective of this proposal is to obtain control data from pregnant rhesus monkeys and to collect blood and fecal samples for analysis. This study will provide a negative control group for a previous study performed at Yerkes that involved challenge of pregnant rhesus macaques with L. monocytogenes during late gestation. In this proposal pregnant monkeys will be given sterilized whipped cream (vehicle) and collected blood and fecal samples at various intervals to ascertain lack of Listeria infection while collecting immune response parameters from blood mononuclear lymphocytes. Both the dam and offspring are returned to the colony after the last blood sample collection.