COBRE: LSU: LIPIDOMIC CORE RESOURCE MODULE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该核心模块为研究脂质信号的实验设计提供指导和建议。该核心还协助和培训Cobre研究人员进行组织细胞采样、处理和提取，然后使用串联液相色谱-光电二极管阵列-电喷雾电离-串联MS/MS脂类分析进行脂类纯化和/或衍生，以确定腐烂的脂类信使的结构特征。高效液相色谱-光电二极管阵列-电喷雾电离-大气压化学电离-串联质谱仪是在最先进的三重四极LC-MS或离子陷阱上进行的，从而提供了强大而灵活的脂类介体的定量分析和结构表征。其他分离技术也是可用的，如高效液相、气相色谱、薄层色谱和固相萃取，以及通过放射、放射免疫分析或荧光应用进行分析。COBRE参与者得到数据处理和解释方面的技术支持和协助。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core module provides guidance and advice for experimental design to study lipid signaling. This core also assists and trains COBRE investigators on tissue cell sampling, processing, and extraction, followed by lipid purification and/or derivation for structural characterization of putattive lipid messengers using tandem liquid chromatography-photodiode array-electrospray ionization-tandem MS/MS-based lipidmoic analysis. Liquid chromatography-photodiode array-electrospray ionization-atmospheric pressure chemical ionization-tandem mass spectrometry is performed on state-of-the-art triple quadrupole LC-MS, or an ion trap, thus providing powerful and flexible quantitative analysis and structural characterization of lipid mediators. other separation techniques are also available, such as HPLC, GLC, TLC, and solid-phase extrction, as well as analysis by radiometric, radio-immunoassay, or fluorometric applications. COBRE participants receive technical support and assistance on data processing and interpretation.