Animal Shared Resource

动物共享资源(ASR)的功能是促进高效、经济和最先进的动物模型用于癌症相关研究。这是通过一个集中的资源实现的，AALAS认证的兽医技术员/技术员为Lombardi成员提供高质量的研究服务。一个重要的重点是使用转基因小鼠，无性系小鼠， 还有斑马鱼。提供的主要服务包括协调动物和特殊动物的饲料购买请求；提供特定操作，包括饮食、激素或药物的投放，以改变肿瘤的膨胀、促进、进展或转移；接种肿瘤细胞或肿瘤细胞系在动物体内的繁殖；投放致癌物质；监测肿瘤生长；收集肿瘤、器官和血清；进行身体解剖和活组织检查；斑马鱼研究，包括向鸡蛋微量注射反义Morpholino寡核苷酸(MO)以击倒特定的感兴趣基因；向鸡蛋中微量注射RNA或cDNA以过表达野生型、突变型和嵌合体基因；毒性/药物的筛选；产生转基因斑马鱼；移植细胞；以及异种移植实验。ASR位于乔治城大学医学院比较医学部(DCM)内。DCM是一个集中式的、AAALAC认证的、在美国农业部注册的动物设施，并在NIH备案了一份批准的保证书。作为全面健康监测计划的一部分，通过使用在每个动物房间的每个架子上饲养的哨兵鼠来监测一般环境和动物健康。都是免疫缺陷 啮齿动物被维持在特定的无病原体环境中。大多数转基因小鼠的群体都保存在专门的屏障隔离设施中。这种资源的服务对于Lombardi研究人员来说至关重要，他们进行临床前研究，使用最先进、适当、有效和经济的动物模型来测试与癌症治疗和预防相关的假设。训练有素和经验丰富的技术人员与Lombardi成员密切合作，为需要使用动物的同行评议的癌症研究提供高质量的动物模型技术服务。2008年，这些服务被用于支持伦巴迪五个癌症中心项目中18名成员的同行评议项目。

The function of Animal Shared Resource (ASR) is to facilitate the efficient, economical, and state-of-art use of animal models for the performance of cancer-related studies. This is accomplished through a centralized resource, where AALAS-certified veterinary technicians/technologists provide high-quality research services to Lombardi members. A significant emphasis is placed on the use of genetically modified mice, athymic mice, and zebrafish. Major services provided include coordination of animal and special animal feed purchase requests; performance of specific manipulations, including diet, hormone or drug administrations to modify tumor inflation, promotion, progression or metastasis; inoculation of tumor cells or in vivo propagation of tumor cell lines in animals; administration of carcinogens; monitoring tumor growth; collection of tumors, organs, and sera; performance of necropsies and biopsies; and zebrafish studies, including microinjecting eggs with antisense Morpholino oligonucleotides (MO) to knock down specific genes of interest; microinjecting eggs with RNA or cDNA to overexpress wild-type, mutant and chimeric genes; screening for toxicity/drugs; generating transgenic zebrafish; transplanting cells; and xenotransplantation experiments. The ASR is located within Georgetown University Medical School's Division of Comparative Medicine (DCM). The DCM is a centralized, AAALAC-accredited, USDA-registered animal facility and has an approved letter of assurance on file at the NIH. The general environment and animal health is monitored through the use of sentinel mice maintained on each rack in each animal room as part of a comprehensive health surveillance program. All immune-deficient rodents are maintained within a Specific Pathogen Free environment. Most genetically modified mice colonies are maintained in a dedicated barrier isolation facility. The services of this resource are critical for the Lombardi investigators who perform preclinical studies to test hypotheses related to treatment and prevention of cancer using state-of-art, and appropriate, effective and economical animal models. The highly trained and experienced technical staff works closely with Lombardi members to provide quality animal model technical services in support of peer reviewed cancer research requiring the use of animals. In 2008, these services were used in support of peer-reviewed projects of 18 Lombardi members in five Cancer Center Programs.