Cell Biology/Morphology Core

细胞生物学/形态学核心为个体研究人员提供与BADERC资助任务相关的细胞生物学、形态学和免疫细胞化学研究的技术支持、设备和专业知识。PI及其同事的专业知识涵盖了广泛的成像程序，从抗原定位的基本和共聚焦光/荧光显微镜分析，到超薄冷冻细胞和组织切片的高分辨率电子显微镜和免疫金标记，以及定量活细胞成像和比例成像。作为一项主要目标，核心将为培训参与实验室的关键人员提供适当的环境。核心的基本原理在于，在一个没有任何一个实验室能够在几个重要和专业领域发展最高水平技术能力的时代，有必要为糖尿病和其他指定研究领域的问题提供综合方法。此外，细胞生物学核心的设计是为了最大限度地利用和提供一些专门的设备，这些设备不仅对许多实验室来说过于昂贵，而且还需要昂贵的定期维护。核心提供的技术要求很高

The Cell Biology/Morphology Core provides individual investigators with technical support, equipment, and expertise for cell biological, morphological and immunocytochemical studies related to the mission of the BADERC grant. The expertise of the PI and his colleagues spans a wide range of imaging procedures, from basic and confocal light/fluorescence microscopic analysis of antigen localization, to high resolution electron microscopy and immunogold labeling on ultrathin frozen sections of cells and tissues, and quantitative live cell imaging and ratiometric imaging. The Core will, as a major objective, provide an environment appropriate for the training of key personnel from participating laboratories. The rationale for the Core lies in the necessity to provide an integrated approach to problems in Diabetes and other designated research areas, in an era when no single laboratory can develop the highest level of technical competence in several important and specialized areas. In addition, the Cell Biology Core is designed to maximize the use and availability of several specialized items of equipment, that are not only prohibitively expensive for many laboratories, but that also require expensive and regular maintenance. The techniques offered by the Core require a high level of sophisticated technology that is constantly being updated to maintain state-of-the-art performance. Thus, investigators are able to explore the use of a varied and constantly expanding arsenal of methods in order to achieve success in their studies. These procedures are an essential part of the modern multidisciplinary approach to cell biological questions as they relate to Diabetes and Endocrinology.