STRUCTURE OF THE U2-U6 COMPLEX

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们正在研究酿酒酵母U2-U6 SnRNA复合体的结构。这种大的RNA复合体在真核生物进化过程中高度保守，并形成剪接体的催化核心。RNA结构将在一系列温度、pH和离子条件下进行研究。我们需要收集的核磁共振实验包括：1D(1H)、2D NOESY、2D 1H-15N HSQC(正常和TROSY增强)、2D 1H-15N HNN COSY、2D 1H-13C HSQC(正常和TROSY增强)、2D NOESY、2D 1H、13C过滤/编辑NOESY。将测量处于各向同性和部分排列状态的样品的剩余偶极耦合(通过添加PF1噬菌体)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are investigating the structure of the U2-U6 snRNA complex from S. cerevisiae. This large RNA complex has been highly conserved throughout eukaryotic evolution, and forms the catalytic core of the spliceosome. The RNA structure will be investigated at a range of temperatures, pH and ionic conditions. NMR experiments that we will need to collect include: 1D (1H), 2D NOESY, 2D 1H-15N HSQC (normal and TROSY enhanced), 2D 1H-15N HNN COSY, 2D 1H-13C HSQC (normal and TROSY enhanced), 2D NOESY, 2D 1H,13C filter/edit NOESY. Residual dipolar couplings will be measured for samples in both isotropic and partially aligned states (via addition of Pf1 phage).