STRUCTURAL STUDIES ON SOLUBLE AND MEMBRANE BOUND PROTEINS INVOLVED IN OXIDATIVE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 甘油代谢途径是一条基本的生化途径，对细胞基本成分的生物合成和能量生产至关重要。在功能上，甘油的摄取依赖于细胞质甘油激酶(GlpK)，它为非ATP依赖的甘油摄取提供化学驱动力。甘油摄取的增强是通过甘油促进剂(GlpF)形成的膜通道来实现的。另一种膜蛋白，甘油-3-磷酸(G3P)脱氢酶(GlpD)，将甘油-3-磷酸氧化为二羟丙酮磷酸，可通过糖酵解途径用于能量生产，并提供线粒体呼吸链的酶III的还原等价物。此外，GlpD可以合成G3P，用于生物合成并入细胞膜的甘油磷脂。GlpD的一个可溶的同系物是甘油磷酸氧化酶(GlpO)，它催化G3P氧化成DHAP的相同反应，也通过G3P穿梭在呼吸中发挥作用，G3P穿梭回收线粒体电子传输链的还原等价物。因此，所有这些酶在生物合成途径和能量生产中都发挥着非常重要的作用。提出了全面的结构研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The glycerol metabolic pathway is a fundamental biochemical pathway, crucial to the biosynthesis of essential cellular components and for energy production. Functionally, the uptake of glycerol relies on the cytoplasmic glycerol kinase (GlpK), which provides the chemical driving force for the non-ATP dependent uptake of glycerol. Enhanced glycerol uptake is mediated through the membrane-channel formed by the glycerol facilitator (GlpF). Another membrane protein, the glycerol-3-phosphate (G3P) dehydrogenase (GlpD), oxidizes glycerol-3-phosphate to dihydroxyacetone phosphate, which can be utilized in energy production via the glycolytic pathway and provides reducing equivalents to Enzyme III of the mitochondrial respiratory chain. Additionally, the GlpD can synthesize G3P for the biosynthesis of the glycerophospholipids that are incorporated into cellular membranes. A soluble homolog of the GlpD is the glycerophosphate oxidase (GlpO), which catalyzes the same reaction of oxidizing G3P to DHAP and also plays a role in respiration through the G3P shuttle that recycles reducing equivalents to the mitochondrial electron transport chain. Consequently, all of these enzymes play highly important roles in biosynthetic pathways and in energy production. Comprehensive structural studies are proposed.