APO-SUPEROXIDE DISMUTASE SOLUTION STRUCTURE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 从抗氧化防御到氧合光合作用，锰氧化还原化学在分子、细胞和环境尺度的生物过程中是基本的。生物医学上重要的酶，如锰超氧化物歧化酶(MnSOD)、锰过氧化氢酶(MNC)和草酸氧化酶(OXO)，其生物学功能需要一个催化的锰辅酶因子，但对其传递必需金属离子的分子机制的研究才刚刚开始。这个项目的目的是促进我们对金属结合成熟的生物化学机制的理解，这种金属酶是普遍存在的和必不可少的抗氧化剂金属酶，MnSOD。这项工作对于理解锰贩运在人类健康和疾病中的作用至关重要。此外，了解这种锰金属酶的金属结合机制可能有助于对氧化应激的生物防御机制的新见解，氧化应激是衰老和神经退行性疾病的关键因素。这项工作也可能对发生金属错结合的代谢稳态疾病有所帮助。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Manganese redox chemistry is fundamental to biological processes at molecular,cellular and environmental scales, from antioxidant defense to oxygenicphotosynthesis. Biomedically important enzymes including Mn superoxide dismutase(MnSOD), Mn catalase (MnC), and oxalate oxidase (OXO) require a catalytic manganesecofactor for their biological function, yet the molecular mechanisms involved indelivering the essential metal ion are just beginning to be studied in detail. The goal of this project is to advance our understanding of the biochemicalmechanisms responsible for the metal-binding maturation of the ubiquitous andessential antioxidant metalloezyme, MnSOD. This work is fundamental to understandingthe role of manganese trafficking in human health and disease. Further,understanding the metal binding mechanism of this Mn metalloenzyme may contributenew insight into biological defenses against oxidative stress, a key factor in agingand neurodegenerative disorders. This work may also shed light on diseases of metalhomeostasis in which metal misincorporation occurs.