STRUCTURAL STUDIES OF K+ CHANNELS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们正在使用的Ca 2+激活的K+通道，称为MthK的X-射线晶体学研究，以更好地理解后的结合的Ca 2+，门控过程的构象变化，和K+传导过程的通道激活的机制。 我们也在结晶另一个配体门控通道，它结合环核苷酸分子以打开和关闭。 结构数据将增加我们对配体结合如何调节离子通道孔的门控机制的理解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are using X-ray crystallography studies of a Ca2+ activated K+ channel, called MthK, in order to understand better the mechanisms of channel activation upon the binding of Ca2+, the gating process conformational change, and the K+ conduction process. We are also crystallizing another ligand gated channel that binds cyclic nucleotide molecules in order to open and close. The structural data will increase our understanding of how ligand binding regulates the gating mechanism of the ion channel pore.