NACL/DNASEI/MNASE RELEASED PROTEINS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 继先前的发现，不同的转录因子（TF）释放 用不同的盐浓度，我试图测试不同盐浓度的梯度， 并查看每一步释放的TF的轮廓。此外，我试图复制一个 来自Henikoff et. 2009年的基因组研究表明， 与MNA酶，并随后进行盐分馏，低盐馏分对应于 人类基因组中的活性转录元件。不幸的是， 我使用的DNA酶不足以消化细胞核，因此DNA酶数据类似于 盐的数据 该项目提供了许多有趣的结果，目前正在使用SRM进行验证， ChIP-seq和SILAC样方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Subsequent to the previous findings that different transcription factors (TFs) are released with different salt concentrations, I sought to test a gradient of different salt concentrations and see the profile of TFs that are released by each step. Additionally, I sought to replicate a finding from Henikoff et. al. Genome Research 2009 that showed that if you treat nuclei with MNAse, and subsequently do salt fractionation, the low salt fractions correspond to active transcriptional elements in the human genome. Unfortunately, the concentration of DNAse that I used was not sufficient to digest the nuclei, so the DNAse data is analogous to the salt data. This project provided many interesting results that are currently being validated using SRM, ChIP-seq and SILAC like approaches.