THE MECHANISM OF DPP TRANSPORTATION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在蝇翼成虫盘中，Dpp梯度从局部来源形成，其中Dpp从邻近前（A）-后（P）隔室边界的前细胞条带分泌。翅盘由一个腔和两层细胞组成，一层为周足上皮，另一层为柱状细胞层。 管腔中的自由扩散不能产生梯度，因为Dpp的分泌转运结构域已经与GFP融合，并且融合GFP不能形成梯度（Entchev等人，2000年）。提出了几种转运机制，包括限制性扩散、平面转胞吞、胞质素和argosomes。我们将通过将民进党标记为 PhotoActivetable-GFP，并用FCS或单粒子跟踪等技术记录Dpp-PAGFP脉冲的运动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the fly wing imaginal disc, a Dpp gradient forms from a localized source, where Dpp is secreted from a stripe of anterior cells that is adjacent to the anterior (A)-posterior (P) compartment boundary. The wing disc consists of a lumen and two layers of cells, a peripodial epithelium and a columnar cell layer. Free diffusion in the lumen can not produce the gradient because the secretory transport domains of Dpp have been fused to GFP, and that fusion GFP fails to form a gradient (Entchev et al., 2000). Several transport mechanisms are proposed involving restricted diffusion, planar transcytosis, cytonemes and argosomes. We are going to address this question by tagging Dpp with PhotoActivetable-GFP, and record the movement of a pulse of Dpp-PAGFP with techniques like FCS or single-particle tracking.