MOUSE BRAIN ATLAS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们之前对活跃染色的小鼠大脑的工作使我们能够看到20微米的细胞结构。但是，我们对这个分辨率水平上的对比度来源的理解是相当原始的。本项目的目标是更全面地了解使用9 T MR磁体的活性染色小鼠脑中的细胞结构的对比度的来源。 1.我们将在40微米处获得小鼠大脑的定量T1和T2图，并伴随更高分辨率的自旋回波（T1加权）图像，以确定细胞结构。 2.我们将对相同的大脑进行组织切片，并将所得数据集配准，以使用尼氏和甲酚紫产生3D光学切片数据。 3. MR和光学切片将配准到单个卷中。 4.所得数据将用于定义活性染色大脑最关键区域中的特定对比度来源。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our previous work with actively stained mouse brains has allowed us to see the cytoarchitecture down to 20 microns. But, our understanding of the sources of contrast at this level of resolution is quite primative. The goal of this project is to understand more fully the sources of contrast of the cytoarchitecture in the actively stained mouse brain using the 9T MR magnet. 1. We will acquire quantiative T1 and T2 maps of the mouse brain at 40 microns with accompanying higher resolution spin echo (T1-weighted) images for definitiation of the cytoarchitecture. 2. We will histologically section the same brains and register the resulting datasets to produce 3D optical sectioned data with Nissl and Cresyl violet. 3. MR and optical sections will be registered into single volumes. 4. The resulting data will be used to define the specific sources of contrast in the most critical regions of the actively stained brain.