MBIRN-MOUSE BIOMEDICAL INFORMATICS RESEARCH NETWORK

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们继续使用MBIRN来集成NIH的4个国家资源的计算内部结构：杜克活体显微镜中心、加州大学洛杉矶分校的神经成像实验室和加州理工大学贝克曼研究所的MRI中心；NCMIR和NBCR-都在加州大学洛杉矶分校。 MBIRN将提高正在进行的神经系统疾病小鼠模型的合作效率： (1)具有多巴胺能系统变化的DAT基因敲除小鼠，使其成为研究精神分裂症、ADHD和药物滥用的理想工具。 (2)EAE小鼠模型(化学诱导和转基因)，表现为多发性硬化症的间歇性虚弱和脱髓鞘特征。MBIRN将能够构建多尺度图像的可伸缩联合数据库，包括MRM、环切的整个大脑、包括定位基因表达的传统组织学、具有蛋白质定位的3D亚细胞数据，以及来自透射电子显微镜和即时高压电子显微镜的超分子图像数据。 (3)工作中开发的基础设施将为推广到其他神经系统疾病的动物模型奠定基础。为这些特定动物模型开发的数据库将提供对这些模型的新见解，并作为BIRN合作伙伴网站小组正在进行的人脑结构成像的非侵入性成像工作的参考数据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We continue MBIRN to integrate computational intrastructure of 4 NIH national resources: Duke Center for In Vivo Microscopy, Lab of neurological imaging at UCLA with MRI center at Caltech's Beckman Institute; NCMIR, and NBCR---both at UCSD. MBIRN will enhance productivity of ongoing collaboration in basic mouse models of neurological disorders: (1) DAT knockout mouse with alterations in dopaninergic system, making it ideal for studies of schizophrenia, ADHD, and substance abuse. (2) EAE mouse models (both chemically induced and transgenic), which undergo episodic weakness and demylination characteristic of multiple sclerosis. MBIRN will enable construction of scalable federated databases of multi-scale images including MRM, cyrosectioned whole brains, conventional histology, including localized gene expression, 3D subcellular data with protein localization, and supramolecular image data from transmission electron microscopy (TEM) and immediate high voltage electron microscopy. (3) Infrastructure developed in the work will lay the foundation for extension to other animal models of neurologic disease. Databases developed for these specific animal models will provide new insight into these models and serve as reference data for non-invasive imaging work being undertaken by BIRN partner site group on human brain structure imaging.