DISPERSION TOMOGRAPHY FOR DNA QUANTIFICATION OF A LIVE CELL

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个项目的主要动机是在不添加任何额外试剂的情况下量化活细胞内的DNA数量。DNA的折射率在紫外光附近没有得到准确的测量，但已报道的吸收光谱表明它具有与蛋白质不同的色散特性。因此，通过测量活细胞在两个或更多不同波长的折射率图，我们期望量化活细胞内DNA的三维分布。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary motivation of this project is to quantify the DNA quantity within a live cell without adding any extra agent. The refractive index of DNA has not been accurately measured near the ultraviolet range, but the reported absorption spectra suggest it has different dispersion characteristics from proteins. Hence, by measuring the refractive index map of a live cell at two or more different wavelengths, we expect to quantify three dimensional distribution of DNA within a live cell.