SKYLINE: AN OPEN SOURCE DOCUMENT EDITOR FOR CREATING AND ANALYZING TARGETED PROT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Skyline是一个Windows客户端应用程序，用于目标蛋白质组学方法创建和定量数据分析。它是开源的，可免费用于学术和商业用途。Skyline用户界面简化了质谱仪方法的开发和使用选择反应监测（SRM）进行的靶向蛋白质组学实验数据的分析。Skyline支持使用和创建来自各种来源的MS/MS光谱库，以选择SRM过滤器并基于先前观察到的离子阱数据验证结果。Skyline将转换列表导出到Agilent、Applied Biosystems、Thermo Fisher Scientific和沃茨三重四极杆仪器，并从这些仪器导入本地输出文件，从而将质谱仪输出无缝连接回实验设计文档。快速紧凑的Skyline文件格式易于共享，即使对于需要多次进样的实验也是如此。丰富的图形显示结果，并提供强大的工具来检查数据采集时的数据完整性，帮助仪器操作员及早发现问题。Skyline动态报表设计器从Skyline文档模型导出表格数据，以便使用常用统计工具进行深入分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Skyline is a Windows client application for targeted pro-teomics method creation and quantitative data analysis. It is open source and freely available for academic and commercial use. The Skyline user interface simplifies the development of mass spectrometer methods and the analysis of data from targeted proteomics experiments performed using Selected Reaction Monitoring (SRM). Skyline supports using and creating MS/MS spectral libraries from a wide variety of sources to choose SRM filters and verify results based on previously observed ion trap data. Skyline exports transition lists to and imports the native output files from Agilent, Applied Biosys-tems, Thermo Fisher Scientific and Waters triple quadrupole instruments, seamlessly connecting mass spectrometer output back to the experimental design document. The fast and compact Skyline file format is easily shared, even for experiments requiring many sample injections. A rich array of graphs displays results and provides powerful tools for inspecting data integrity as data is acquired, helping instrument operators to identify problems early. The Skyline dynamic report designer exports tabular data from the Skyline document model for in-depth analysis with common statistical tools.