A novel desensitizing paste containing enamel-like crystals

描述(申请人提供)：牙本质过敏症是一个常见的问题，治疗方式是短暂的，如果它们真的奏效的话。本项目的目标是生产一种能够封闭牙本质小管开放的产品(牙本质小管形成的原因)，并能附着在牙本质小管潮湿的内表面上。本研究的研究目标是优化氟磷灰石(FA)晶体/聚合物的粘附性聚合物、聚(DMA-MEA)的比例，以达到即刻和长期封闭牙本质小管的目的。FA晶体与牙釉质和合成聚合物中发现的相同，后者模仿贻贝粘液中发现的裸露氨基酸3，4-二羟基苯丙氨酸(DOPA)。将合成的FA晶体与聚合物按一定比例混合，应用于牙本质切片，测量牙本质电导率(用于测试牙本质脱敏剂的金标准)。。一旦确定了最佳的咬合比例，将在扫描电子显微镜下观察其对牙本质小管闭塞的影响。在中性和酸性的pH条件下，还将测量F、Ca和PO4从FA/聚合物糊中的释放。这种离子释放和唾液一起，将有助于在牙本质上形成一层矿化层，这将有助于长期缓解牙周炎。这款用于治疗牙周炎的产品将是：生物相容、无毒、允许离子扩散；易于使用；在口腔潮湿的环境中附着于牙齿结构；以及美观--一种“天然”产品。 与公共卫生相关：这笔赠款提出了一种治疗敏感牙齿的新方法；这个问题有时会影响到大约50%的人口。一种脱敏膏将被生产出来，它含有类似于牙齿中天然晶体的矿物晶体，与将粘在潮湿的牙齿表面的凝胶混合在一起。预计这种糊剂将通过阻止从牙本质表面到牙根活体部分的开放小管而立即缓解，并通过在敏感的牙齿表面形成矿化屏障来长期缓解。

DESCRIPTION (provided by applicant): Dentin hypersensitivity (DH) is a common problem and the treatment modalities are, if they work at all, short lived. The object of this project is t produce a product that can occlude patent dentinal tubules (the cause of DH) and adhere to the wet interior surface of the dentinal tubules. The research goal of this study is to optimize the fluorapatite (FA) crystal/polymer of adhesive polymer, poly(DMA-MEA) ratio for immediate and long term occlusion of the patent dentinal tubules. The FA crystals are the same as those found in dental enamel and synthetic polymer which mimics the naked amino acid 3,4 dihydroxyphenylalanine (DOPA) found in the mussel adhesive mucus. The synthesized FA crystals will be mixed with the polymer at certain ratios, applied to dentin sections and the dentinal conductivity (the gold standard used for testing dentin desensitizing agents) measured. . Once the optimized ratio for occlusion has been established its effect on dentinal tubule occlusion will be observed in the SEM . The release of F, Ca and PO4 from the FA/polymer paste will also be measured at neutral and acidic pH. This ion release, along with saliva, will help in the formation of a mineralized layer on the dentin which will help in the long term relief f DH. This product for treating DH will be: biocompatible, non-toxic, allow for diffusion of ions; easily applied; adherent to tooth structure in the wet environment of the oral cavity; and aesthetic - a "natural" product. PUBLIC HEALTH RELEVANCE: This grant proposes a novel way of treating sensitive teeth; a problem that affects about 50% of the population at sometime. A desensitizing paste will be produced which contains mineral crystals which are similar to the natural crystals in teeth, mixed with a gel that will stick to wet tooth surfaces. It is anticipated that the paste will give immedite relief by blocking open tubules running from the surface of the dentin to the live part of the toot; and long term relief by causing a mineralized barrier to form, overtime, on the sensitive tooth surface.