Bioanalytical Laboratory

使命：生物分析实验室（BL）是综合癌症研究所的一个大量使用的共享资源。 中心的使命是提供先进的分析服务、技术和科学 DNA、蛋白质和脂质化学咨询。BL专门从事发现，鉴定， 生物分子的表征和定量，包括：蛋白质、肽和 脂质的质谱和生物化学方法; DNA序列分析; DNA/RNA合成; 蛋白质、肽、DNA和脂质的色谱分析;以及组织中的临床生物标志物的定量， 血液样本 资产：BL的资产包括10多个主要仪器系统和员工的专业知识 成员共同董事，博士马克莱弗利和迈克尔托马斯，是专家化学家与超过50年 在研究和核心实验室管理方面的综合经验。托马斯医生是个脂肪团 光谱学专家莱弗利博士是一个创始成员和协会的前主席， 生物分子资源设施（ABRF）。他是国家咨询研究资源的成员 NIH国家研究资源中心理事会。BL技术人员拥有80多年的 结合实验室经验。 用途：BL支持了2009年11月至2010年10月73名中心成员的研究，分析了10，234份 样品使用最多的服务是：质谱法（4 018个spls）; DNA测序 （4，299 spI）;生物标志物HPLC（746 spI）;蛋白质/肽方法（248 spI）;和DNA合成（182 spI）。 中心成员获得了60%的服务。 未来方向：BL最近增加了两个重要的新质谱仪，一个ThermoFisher TSQ Discovery Max电喷雾，三重四极杆质谱仪，用于增强极性脂质的研究， ThermoFisher TSQ Quantum XLS三重四极杆气相色谱（GC）质谱仪。建议 为蛋白质组学的Q-TOF质谱仪获取Nanomate源。这些文书 允许BL提供脂质、蛋白质和肽的新的和改进的MS服务。临床生物标志物 分析服务将扩大，以加强对正在进行的临床研究的支持。

Mission: The Bioanalytical Laboratory (BL) is a heavily used shared resource of the Comprehensive Cancer Center with a mission to provide access to advanced analytical services, technologies and scientific consultation for DNA, protein, and lipid chemistry. The BL specializes in discovery, identification, characterization, and quantification of biomolecules including: structural analysis of proteins, peptides and lipids by mass spectrometry and biochemical methods; DNA sequence analysis; DNA/RNA synthesis; chromatography of proteins, peptides, DNA, and lipids; and quantification of clinical biomarkers in tissue and blood specimens. Assets: Assets of the BL include more than 10 major instrument systems and the expertise of its staff members. Co-directors, Drs. Mark Lively and Michael Thomas, are expert chemists with more than 50 years of combined experience in research and core laboratory management. Dr. Thomas is a lipid mass spectrometry expert. Dr. Lively is a founding member and former president of the Association of Biomolecular Resource Facilities (ABRF). He is a member of the National Advisory Research Resources Council of the NIH National Center for Research Resources. The BL technicians have more than 80 years of combined laboratory experience. Usage: The BL supported the research of 73 Center members from 11/2009 -10/2010, analyzing 10,234 samples. The most heavily used services were: mass spectrometry methods (4,018 spls); DNA sequencing (4,299 spIs); biomarker HPLC (746 spIs); protein/peptide methods (248 spIs); and DNA synthesis (182 spIs). Center members received 60% of the services performed. Future directions: The BL recently added two important new mass spectrometers, a ThermoFisher TSQ Discovery Max electrospray, triple quadrupole mass spectrometer, to enhance the study of polar lipids and a ThermoFisher TSQ Quantum XLS triple quadrupole gas-chromatograph (GC) mass spectrometer. An Advion Nanomate¿ source was acquired for the Q-TOF mass spectrometer for proteomics. These instruments permit the BL to provide new and improved MS services of lipids, proteins, and peptides. Clinical biomarker analysis services will be expanded to enhance support of ongoing clinical studies.