MOLECULAR BIOLOGY AND TISSUE ENGINEERING CORE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 子项目说明 分子生物学（MBTE）核心设施由过桥资金资助，继续通过进行分子和细胞生物医学研究所需的工具、技术和服务为研究人员提供研究支持。我们正在请求RCMI计划继续提供过渡资金支持，以便MBTE核心设施继续运作，同时正在准备RCMI续期申请，以供2011年5月提交。MBTE核心设施将是新的RCMI竞争性续期申请的一部分。MBTE核心设施资源对于我们新的RCMI计划的目标至关重要，该计划专注于基因组，表观基因组和蛋白质组的高级分子分析，这将有助于理解疾病发病机制和促进药物发现和开发，最终通过跨学科科学家群体的聚集进行转化研究，因此，为我们的机构生物医学和转化研究的愿景和使命做出贡献。因此，MBTE核心基金的具体目标是： 具体目标1：继续为哺乳动物细胞培养、组织外植体培养和细菌培养提供资源、仪器、技术和实践支持服务。 具体目标#2：继续为不同的分子技术提供工具、培训和技术支持服务，包括常规和实时PCR、低密度DNA阵列分析、RNA干扰、microRNA、启动子-报告基因分析、染色质免疫沉淀（ChIP）分析、细胞内蛋白质分析、表观遗传修饰和信号通路分析。 具体目标#3：通过研讨会、期刊俱乐部会议和研究报告等研究开发活动，继续为大津的研究人员创造一个激励和支持的科学氛围。 具体目标#4：继续加强合作研究努力，并与大津和其他研究密集型大学的研究人员交流技术能力;并参加技术讲习班。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. SUBPROJECT DESCRIPTION The Molecular biology (MBTE) core facility, which is funded through bridge funding, continues to provide research support to investigators through tools, techniques and services required to perform molecular and cellular biomedical research. We are requesting continued bridge funding support from the RCMI Program for the continued functioning of the MBTE core facility, while the RCMI renewal application is being prepared for submission in May 2011. The MBTE core facility will be part of the new RCMI competitive renewal application. The MBTE core facility resources are vital to the goals of our new RCMI program focusing on advanced molecular analysis of genomes, epigenomes and proteomes, which will assist in the understanding of disease pathogenesis and promotion of drug discovery and development, eventually leading to translational research through the congregation of interdisciplinary groups of scientists and thus, contributing to our institutional biomedical and translational research vision and mission. Therefore, the specific aims of the MBTE core facility are to: Specific Aim #1: Continue to provide resource, instrumentation, and technical and hands-on support services for mammalian cell culture, tissue explant culture and bacterial culture. Specific Aim #2: Continue to offer tools, training and technical support services for different molecular technologies including conventional and real-time PCR, low density DNA array analysis, RNA interference, microRNA, promoter-reporter analysis, chromatin immunoprecipitation (ChIP) analysis, in-cell western analysis, epigenetic modifications and analysis of signaling pathways. Specific Aim #3: Continue to create a stimulating and supportive scientific atmosphere for the researchers at TSU through research development activities such as seminar programs, journal club meetings, and research presentations. Specific Aim #4: Continue to increase collaborative research efforts, and pursue exchange of technical capabilities with investigators at TSU and other research-intensive universities; and by attending technology workshops.