Nonhuman Primate Contraceptive Core

项目总结（见说明）： 非人灵长类避孕核心（NPC核心）为U 54避孕中心的研究人员提供服务和动物使用的协调。NPC核心在ONPRC动物资源部（DAR）维持一个食蟹猴雌性和雄性样本库，可用于避孕（交配）试验;协调并开展潜在避孕药研究;确认 她还可以通过阴道拭子检查来确定是否已经发生了交配;进行超声波检查以监测怀孕情况;并获取血液样本用于激素测定、药代动力学研究和代谢概况。核心使雌性适应小群体（后宫）的居住环境（10只雌性对1只雄性），其中它们可以分开进行每日阴道检查、给药、血液采样和超声检查 分析。此外，全国方案协调中心还就既定的方案对调查人员进行培训，并传播关于新方法的信息。NPC核心代表了对动物和人员的有效利用，并提供了在个体研究者实验室中建立的昂贵的援助。 发现用于女性生育调节的新型药物的策略的最后阶段将是在非人灵长类动物模型中评价卵巢/生殖道特异性药物作为可逆或不可逆避孕药的疗效。U 54中每个项目的这些抑制剂的成本效益、实验性避孕试验将在NPC核心中进行，具体目标如下： 具体目标1：在表现出自然月经周期的猕猴的繁殖群体中证明长期（6个月）施用该抑制剂的避孕效果。 具体目标二：记录在停药6个月后避孕效果是否可逆。在NPC Core中进行的这些转化实验的数据将增加考虑将类似的抑制剂方案用作女性避孕药的可行性的信心。

PROJECT SUMMARY (See instructions): The Nonhuman Primate Contraceptive Core (NPC Core) provides services and coordination of animal usage for U54 Contraceptive Center investigators with approved projects. The NPC Core maintains a pool of cynomolgus macaque females and males in the Division of Animal Resources (DAR), ONPRC, available for contraceptive (mating) trials; coordinates and performs studies on potential contraceptive agents; confirms menstruation and ensures mating has occurred by taking vaginal swabs; performs ultrasounds to monitor for pregnancies; and obtains blood samples for hormone assays, pharmacokinetic studies and metabolic profiles. The Core acclimates the females to a small group (harem) housing situation (10 females to one male) wherein they can be separated for daily vaginal checks, dosing, blood sampling and ultrasound analyses. In addition, the NPC Core trains investigators in established protocols and disseminates information on emerging approaches. The NPC Core represents an efficient use of animals and personnel, and provides assistance that is prohibitively expensive to establish in individual investigator's laboratories. The final stage in the strategy to discover novel agents for female fertility regulation will be to evaluate the efficacy of ovary/reproductive tract-specific agents as reversible or irreversible contraceptive agents in a nonhuman primate model. Cost-effective, experimental contraceptive trials of these inhibitors for each individual project in the U54 will be performed in the NPC Core with the following specific aims: Specific Aim 1: demonstrate a contraceptive effect of chronic (6 month) administration of the inhibitor in a breeding colony of macaques exhibiting natural menstrual cycles. Specific Aim 2: document if the contraceptive effect is reversible following discontinuation for a 6 month interval Data from these translational experiments conducted in the NPC Core will increase confidence in the feasibility of considering a similar regimen of inhibitor(s) for clinical use as a contraceptive in women.