BIOMARKERS CORE

烟草成分和生物标志评估核心将为项目1-3提供独特的资源。 这个核心的研究人员组成了世界领先的化学学术实验室之一 烟草成分及相关代谢物和生物标志物的分析。下列尿液代谢物 将被量化：尼古丁当量(尼古丁、可替宁和反式-3‘-羟基可替宁及其 葡萄糖醛酸苷)，总NNAL(4-(甲基亚硝基)-1-(3-吡啶)-1-丁醇及其葡萄糖醛酸苷的总和)， 总NNN(游离和糖醛酸化的A/‘-亚硝基或尼古丁的总和)，挥发性的硫代尿酸 致癌物1，3-丁二烯、苯、丙烯醛、巴豆醛和环氧乙烷，以及炎症 和氧化损伤生物标志物前列腺素E酸(11-a-hydroxy-9，15-dioxo-2，3，4，5-tetranorprostane-1，20-dioic ACID) M)和9，11，15-三羟基前列腺素-5，13-二烯-1-酸。我们还将对甲醛-DNA加合物进行量化， /V^-羟甲氧基腺苷，尿中有少量烟草生物碱。这些 生物标志物将为项目1-3中正在进行的研究提供关键信息。 相关性(请参阅说明)： 实现减少使用烟草产品对公共健康危害的方法对 减少烟草使用造成的人类癌症死亡。在这个岩心中被量化的生物标志物是 许多已知的主要致癌和促癌成分的代表 烟草产品。这些生物标志物的量化将为烟草的评价提供重要的工具 以确定其对公众健康的潜在影响。

The Tobacco Constituent and Biomarker Assessment Core will provide a unique resource for Projects 1-3. Investigators in this Core comprise one of the leading academic laboratories in the world for chemical analysis of tobacco constituents and related metabolites and biomarkers. The following urinary metabolites will be quantified: nicotine equivalents (the total of nicotine, cotinine, and trans-3'-hydroxycotinine and their glucuronides), total NNAL (the total of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and its glucuronides), total NNN (the total of free and glucuronidated A/'-nitrosonornicotine), mercapturic acids of the volatile carcinogens 1,3-butadiene, benzene, acrolein, crotonaldehyde, and ethylene oxide, and the inflammation and oxidative damage biomarkers 11-a-hydroxy-9,15-dioxo-2,3,4,5-tetranorprostane-1,20-dioic acid (PGE- M) and 9,11,15-trihydroxyprosta-5,13-dien-1-oic acid. We will also quantify the formaldehyde-DNA adduct, /V^-hydroxymethyideoxyadenosine in leukocyte DNA, and minor tobacco alkaloids in urine. These biomarkers will provide crucial information for the research being carried out in Projects 1-3. RELEVANCE (See instructions): Methods for achieving reduction in public health harm from use of tobacco products are critical for decreasing the human cancer toll of tobacco use. The biomarkers being quantified in this core are representative of many of the major carcinogenic and tumor enhancing constituents known to be present in tobacco products. Quantifying these biomarkers will provide an important tool for the evaluation of tobacco products to determine their potential impact on public health.