Molecular Biology/Gene Expression Core

请参阅说明)： 分子生物学/基因表达核心设施将提供关键服务，以支持科学 该计划项目的目标：1)试剂库-包含特定的质粒、表达构建体、报告 构建、抗体、RNA样本和逆转录病毒/腺病毒/慢病毒载体 食道癌的发生。因此，这些试剂得到了组织和集中。此外, 我们将继续储存新的可用的试剂；2)图像分析和基因表达量化- 有多种仪器可用，例如(但不限于)磷光成像、Gel和Chemidoc、安捷伦 生物分析仪和奥德赛红外蛋白质成像系统、实时定量聚合酶链式反应和转录 DyneX和Orion发光计的分析；3)基因芯片的基因图谱服务(补贴) (Affymetrix平台)，通过宾夕法尼亚微阵列设施(鲍德温博士)和宾夕法尼亚生物信息学设施 (托拜厄斯博士纳入生物统计学核心)；4)具有食道组织的人类组织库 临床组织成分资料库和注释；5)食道细胞系资料库(小鼠和人) 原代、永生化、转化和新基因工程的食道细胞系，供 项目；6)食道组织、细胞系(未转化)的食道数据库储存库(基于网络) 以及转化的有机培养物、微阵列和通用的共享试剂/设备(来自岩心 C和D)。分子生物学/基因表达核心提供质量保证、质量控制、成本- 其服务的有效性、及时性和效率。所有与人体组织有关的成分都是IRB 在患者知情同意和HIPAA合规的情况下获得批准，并携带患者取消身份识别符。此核心 与所有其他核心密切合作，特别是形态核心C，以避免重复和提供 对项目采取协调一致的方法。这些项目从这一核心中受益匪浅， 相互关联的假设和具体目标。此外，此核心在响应不断演变的P01时是动态的 需求，预测未来的P01方向，并整合新兴技术。 相关性(请参阅说明)：

Seeinstructions): The Molecular Biology/Gene Expression Core Facility will provide critical services to support the scientific goals of this Program Project: 1) Reagent Bank- contains specific plasmids, expression constructs, reporter constructs, antibodies, RNA samples, and retroviral/adenoviral/lentiviral vectors of relevance to the study of esophageal carcinogenesis. As a result, these reagents have been organized and centralized. In addition, we will continue to store newly available reagents; 2) Image Analysis and Gene Expression Quantification- multiple instruments are available, such as (but not limited to) Phosphorimaging, Gel and Chemidoc, Agilent Bioanalyzer and Odyssey Infrared Protein Imaging System, real-time quantitative PCR, and transcriptional analysis for Dynex and Orion Luminometers; 3) Gene Profiling Services (subsidized) with gene microarrays (Affymetrix platform) through the Penn microarray facility (Dr. Baldwin) and the Penn bioinformatics facility (Dr. Tobias integrated within the Biostatistics Core); 4) Human Tissue Bank with an esophageal tissue repository and annotated clinical tissue component; 5) Esophageal Cell Line Bank (murine and human) with primary, immortalized, transformed and newly genetically engineered esophageal cell lines for use by the Projects; 6) Esophageal database repository (web-based) for esophageal tissues, cell lines (nontransformed and transformed, organotypic cultures, microarrays, and common, shared reagents/equipment (from Cores C and D). The Molecular Biology/Gene Expression Core provides quality assurance, quality control, cost- effectiveness, timeliness and efficiency in its services. All components that pertain to human tissues are IRB approved with patient informed consent and HIPAA compliance, and carry patient de-identifiers. This Core works closely with all the other Cores, especially the Morphology Core C to avoid duplication and furnish coordinated approaches for the Projects. The Projects benefit tremendously from this Core in advancing their interrelated hypotheses and Specific Aims. In addition, this Core is dynamic in responding to evolving P01 needs, anticipating future P01 directions, and integrating emerging technologies. RELEVANCE (Seeinstructions):