Orbitrap Mass Spectrometer

描述（由申请人提供）：本提案是为南卡罗莱纳医科大学的质谱分析设施增加一台Thermo Scientific LTQ Orbitrap Velos ETD混合FT质谱计。该设施拥有39年的历史，提供最先进的质谱分析能力，促进了当地研究界广泛的生物医学研究兴趣。在过去的三年中，该设施为大学内77名不同的教师调查员和其他机构的多位nih资助的调查员提供了质谱分析和协助。该设施提供的最受欢迎的服务是蛋白质鉴定和翻译后修饰的表征。为此，该设施中使用最多的仪器是低分辨率线性离子阱质谱仪。该设施缺乏LTQ Orbitrap Velos ETD质谱仪可实现的灵敏度、分辨率和多肽碎片多模式的仪器。事实上，在南卡罗莱纳没有Orbitrap质量分析仪。该仪器质量精度的提高将为多肽和蛋白质鉴定以及翻译后修饰（PTMs）的位点分配提供更高水平的信心。将多个片段选择（CID、HCD、ETD）与高质量精度测量相结合，将使定量方法能够同时识别和定量蛋白质修饰，这是目前设备用户无法获得的能力。ETD电离源附件将1)有助于需要表征PTMs和从头测序的项目，2)提供新的功能，可以对在CID过程中被破坏的修饰进行自上而下的蛋白质分析和定量。所要求的仪器将服务于主要用户的代表性群体，其中包括11名美国国立卫生研究院资助的研究人员，他们在癌症、心血管疾病、糖尿病、视力、骨质疏松症和肾脏疾病等领域进行研究。Orbitrap Velos ETD仪器集成了两个质量分析仪和多个碎片化策略，产生了一个功能强大且非常通用的仪器，将非常适合于解决MUSC和南卡罗来纳邻近大学和研究中心的研究人员所要求的广泛的蛋白质分析和表征。

DESCRIPTION (provided by applicant): This proposal is for the addition of a Thermo Scientific LTQ Orbitrap Velos ETD hybrid FT mass spectrometer to the Mass Spectrometry Facility at the Medical University of South Carolina. The facility has a 39 year history of offerin state of the art mass spectrometry capability advancing a wide range of biomedical research interests in the local research community. Over the past three years, the facility has provided mass spectrometric analysis and assistance to 77 different faculty investigators within the university and multiple NIH-funded investigators at other institutions. The most highly requested services offered by the facility are protein identification and the characterization of posttranslational modifications. For these purposes, the most heavily used instruments in the facility are the low resolution linear ion trap mass spectrometers. The facility lacks an instrumen with the combined sensitivity, resolution, and multiple modes of peptide fragmentation that are achievable with the LTQ Orbitrap Velos ETD mass spectrometer. In fact, there is not an Orbitrap mass analyzer in South Carolina. The improved mass accuracy of this instrument will provide a higher level of confidence in peptide and protein identifications and in the site-assignment of posttranslational modifications (PTMs). Combining multiple fragmentation options (CID, HCD, ETD) with high mass accuracy measurements will enable quantitative approaches aimed at simultaneous identification and quantitation of protein modifications, a capability which is not currently available to facility users. The ETD ionization source accessory will 1) aid in projects requiring the characterization of PTMs and de novo sequencing and 2) provide new capabilities enabling top to middle-down protein analysis and quantitation of modifications that are destroyed during CID. The requested instrument will serve a representative group of Major Users including eleven NIH-funded investigators conducting research in the areas of cancer, cardiovascular disease, diabetes, vision, osteoporosis, and kidney disease. The Orbitrap Velos ETD instrument incorporates two mass analyzers and multiple fragmentation strategies yielding a powerful and very versatile instrument that will be well suited to address the broad range of protein analyses and characterization requested by investigators at MUSC and the neighboring universities and research centers in South Carolina. PUBLIC HEALTH RELEVANCE: The analysis of proteins by mass spectrometry is a fundamental analytical method utilized in biomedical research aimed at understanding processes underlying the onset and progression of disease and developing new therapeutics and diagnostics. The requested instrumentation will enhance our ability to identify and monitor changes in protein expression and regulatory post-translational modifications thereby advancing research in multiple areas including cancer, cardiovascular disease, diabetes, blindness, neurological disease, and osteoporosis.