DEVELOPMENT OF A PRIMATE GENOMICS RESOURCE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该模型的主要目标与原始协议中的目标相同。该项目专注于创建基因组/蛋白质组资源，这些资源可以应用于使用非人类灵长类动物的研究。通过蛋白质组资源的建设，我们打算为研究界提供将功能基因组学技术应用于非人类灵长类研究所需的完整的物种特定基因组和蛋白质组工具。我们将使用灵敏、高分辨率的质谱学来建立恒河猴(Macaca Mulatta)各种组织中存在的已识别蛋白质的数据库。这些数据库将被用来表征因猴免疫缺陷病毒感染而发生的细胞蛋白图谱的变化。这些研究将使我们能够在一个实验系统中评估这些资源，该系统将加强使用猕猴作为艾滋病研究的模型。结果将通过门户网站共享，作为一种资源，使研究人员能够最大限度地利用从这些有价值的研究动物那里获得的信息类型和数量。 我们将对一种急性粘膜感染模型进行系统生物学研究，采用直肠内SIVmac251对24只雄性印度猕猴进行攻击。主要的假设是，对SIV致病毒株急性感染早期变化的详细系统水平分析将揭示先天免疫、炎症过程以及随后向适应性免疫反应的转变。作为这一假设的一个推论，这些特征可以作为结果的预测指标，反映在单个动物的可变性上。第二个推论是，这样的系统水平分析将确定药理学药物或疫苗模式干预的特定目标和途径。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The primary goal of the model remains the same as in the original protocol. This project is focused on creating genomic/proteomic resources that can be applied to research using nonhuman primates. Through the construction of proteomic resources, we intend to provide the research community with the full complement of species-specific genomic and proteomic tools needed to apply the technologies of functional genomics to nonhuman primate research. We will use sensitive, high-resolution mass spectrometry to build databases of identified proteins present in various rhesus macaque (Macaca mulatta) tissues. These databases will be used to characterize the changes in cellular protein profiles that occur in response to simian immunodeficiency virus infection. These studies will allow us to evaluate these resources in an experimental system that will enhance the use of macaques as models for AIDS research. The results will be shared via Web portals, as a resource to enable researchers to maximize the types and amounts of information that can be obtained from these valuable research animals. We will perform a systems biology interrogation of an acute mucosal infection model, employing intra-rectal challenge with SIVmac251 in 24 male, Indian-origin rhesus macaques. The primary hypothesis is that a detailed systems level analysis of early changes in the acute infection with a pathogenic strain of SIV will reveal events in innate immunity, inflammatory processes, and the ensuing transition to the adaptive immune response. As one corollary to this hypothesis, these features may serve as prognosticators of outcome, as reflected in the variability of individual animals. A second corollary is that such a systems level analysis will identify specific targets and pathways for intervention by pharmacologic agents or vaccine modalities