NEUTROPENIA FOLLOWING G-CSF ADMINISTRATION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在G-CSF给药后不久，中性粒细胞从循环中急剧但短暂地消失。G-CSF给药后中性粒细胞从循环中一过性丢失似乎是由于中性粒细胞在肺和脾组织内蓄积所致。使用标记中性粒细胞和单核细胞的髓样/组织细胞抗原克隆Mac 387、内皮标记物抗小窝蛋白-1-Cy 3抗体和核染色剂（To-Pro3）的详细共聚焦显微镜显示，中性粒细胞被拉长，似乎被激活，并且紧密粘附于肺血管的内皮。这些结果表明，G-CSF给药后的一过性中性粒细胞减少症与肺和脾组织内中性粒细胞的蓄积有关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Shortly following G-CSF administration there is a dramatic yet transient disappearance of neutrophils from the circulation. The transient loss of neutrophils from the circulation following G-CSF administration appeared to be due to an accumulation of neutrophils within the pulmonary and splenic tissues. Detailed confocal microscopy using a myeloid/histiocyte antigen clone Mac387 which labels neutrophils and monocytes, an endothelial marker anti-caveolin-1-Cy3 antibody, and a nuclear stain (To-Pro3) showed that the neutrophils were elongated, appeared activated, and were tightly adhered to the endothelium of the pulmonary vasculature. These results suggest that the transient neutropenia following G-CSF administration is associated with an accumulation of neutrophils within the pulmonary and splenic tissues.