miRNA profiling of prostate cancer.

我们的目的是研究前列腺肿瘤和正常前列腺上皮组织中miRNA的表达谱，以评估不同分化程度和不同生物学行为的肿瘤中特异性miRNA表达谱是否存在差异。将对前列腺癌进行手动显微切割，以获得纯肿瘤细胞群以及远离肿瘤区域的可用正常上皮。组织学特征，如Gleason评分，神经周围，淋巴管浸润，囊外或精囊浸润的存在也进行了评估和记录每例。差异表达的miRNA将被定义为具有两倍变化（上升或下降）且p值0.05的那些。不同的miRNA表达谱将包括低级别肿瘤（Gleason评分6和7）与高级别（Gleason评分8和9）病变之间的比较。然后，将每个肿瘤组与其相应的正常上皮组进行比较。

Our goal is to investigate the miRNA profiles of prostate tumors and the normal epithelium to evaluate if there are differences in specific miRNA expression patterns of tumors with different grades of differentiation and different biological beavior. Prostate cancers will be manually microdissected to obtain pure populations of tumor cells as well as available normal epithelium distant to the tumor area. Histological features such as Gleason score, presence of perineural, lymphatic invasion, extracapsular or seminal vesicle invasion were also evaluated and recorded for each case. Differentially expressed miRNAs will be defined as those with a two-fold change (up or down) and a p value <0.05. Different miRNAs expression profiles will include comparison between the low grade tumors (Gleason score 6 and 7) vs. high grade (Gleason score 8 and 9) lesions. Then, each tumor group will be compared with their respective group of normal epithelium.