THE ROLES OF PROHIBITINS (PHBS) IN 3T3-L1 ADIPOCYTE DIFFERENTIATION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 A.具体目标 本研究的目的是确定抑制素在3T3-L1脂肪细胞分化过程中的功能意义。我们的初步数据显示，在3T3-L1前脂肪细胞的脂肪细胞分化过程中，这两种抑制素(PHBs)，包括PHB1和PHB2(雌激素受体活性抑制因子，REA)，都显著增加。此外，siRNA-PHB1或siRNA-REA同时下调PHBs蛋白后，3T3-L1脂肪细胞的分化受到抑制。因此，我们的首要目标是确定PHB1和PHB2是否是促进小鼠3T3-L1脂肪细胞分化的关键因素。我们的第二个目的是研究PHB1和PHB2在3T3-L1细胞脂肪形成过程中的信号通路。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A. Specific Aims The goal of this proposal is to determine the functional significance of prohibitins during 3T3-L1 adipocyte differentiation. Our preliminary data showed that both of these prohibitins (PHBs), including PHB1 and PHB2 (repressor of estrogen receptor activity, REA), were significantly increased during the adipocyte differentiation of 3T3-L1 preadipocytes. Moreover, 3T3-L1 adipocyte differentiation was impaired after the simultaneous knockdown of either PHBs protein by siRNA-PHB1 or siRNA-REA. So, our first aim is to determine whether PHB1 and PHB2 are critical factors in promoting mouse 3T3-L1 adipocyte differentiation. Our second aim is to investigate the signaling pathway of PHB1 and PHB2 during 3T3-L1 cell adipogenesis.