COMPUTATIONAL IMAGING CORE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 预防性成像核心 该核心设施提供光学显微镜，全载玻片扫描，体视学，数字成像，图像数据库开发和咨询服务，主要为CNPRC，但也为所有校园部门充值。我们的目标是帮助教师，工作人员和学生与他们的定性和定量成像应用的研究需求。该核心协助制作出版质量的图像，并提供有关其设备使用的实验方法的咨询。 此外，它还从事数字化和校正来自各种来源的存档电影图像，但主要是CNPRC病理学服务创建的图像。 Core还提供注释服务，并将完全校正的数字图像上传到CNPRC图像数据库中。 在数据库中，标记的图像与特定动物的其他信息相链接，并提供给所有获准访问的人。 对于经过培训的CNPRC用户来说，堆芯中的仪器设备每周7天、每天24小时都可以使用。咨询，培训和支持服务，沿着与访问其他部门的设备，可从8-5周一至周五。 该设施容纳以下设备： + 用于冷冻切片的低温恒温器。 + 石蜡切片机和塑料切片机。 + 用于塑料加工和包埋的样品制备设备。 + 数据存储服务器（可扩展的多TB卷），用于存档客户端、病理单元和成像核心数据。 这些系统提供了快速访问非常大的（多千兆字节）文件或数据集，否则需要几个小时才能从CNPRC的其他地点转移。 + 基于网络的仪器预订日历。 + Olympus BX 61研究显微镜，配有1个彩色和2个B/W摄像头、Olympus DSU旋转盘共聚焦系统、计算机控制载物台和聚焦系统、Molecular Devices MetaMorph图像捕获和分析软件以及用于体视学和图像数据收集的Intelligent Imaging Innovations SlideBook软件。 + 第二台Olympus BX 61显微镜配备Intelligent Imaging Innovations SlideBook软件，用于Core员工的体视学技术开发。 + 图形工作站、平板扫描仪和高质量彩色校正打印机;这些用于出版质量打印、将胶片转换为数字图像、图像校正和图像存档。 + 文档扫描仪用于快速数字化和OCR转换纸质记录。 + Delta Vision显微镜系统和计算机工作站，用于非常高分辨率的多维荧光成像和反卷积。 + 用于体视学数据离线分析和大型多维图像分析的计算机工作站。 + 最近更新的Visiopharm维斯/CAST网格体视学系统，用于定量数据收集和自动图像分析。 + Adobe Photoshop、Adobe Illustrator和Adobe LightRoom软件，支持Core的所有数字成像工作  所有电脑均配备彩色平衡显示器。 + 一种荧光体视显微镜，配备有与计算机系统耦合的高灵敏度彩色摄像机。 + 双头明视场体视显微镜 + 用于明场、DIC和荧光成像的Olympus VS 110虚拟显微镜/全载玻片扫描系统 + Cell Biosciences FluorChem-E凝胶和印迹扫描仪

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. COMPUTATIONAL IMAGING CORE This Core facility provides light microscopy, whole slide scanning, stereology, digital imaging, image database development, and consultation services, primarily for the CNPRC, but also for all campus departments on a recharge basis. The goal is to assist faculty, staff, and students with their research needs for qualitative and quantitative imaging applications. This Core assists with the production of publication quality images and offers consultation on experimental approaches for use with its equipment. Additionally, it is engaged in digitizing and correcting archived film images from various sources but primarily those created by the Pathology Service at the CNPRC. The Core also provides a service to annotate and upload fully corrected digital images into the CNPRC image database. Uploaded images are linked in the database to other information for a specific animal and made available to all who have been granted access. Instrumentation housed in the Core is available 24 hours a day, 7 days a week for trained CNPRC users. Consultation, training, and support service, along with access to the equipment for other departments, is available from 8-5 Monday through Friday. The facility houses the following equipment: + A Cryostat for frozen sections. + Microtomes for paraffin and plastic sectioning. + Sample preparation equipment for plastic processing and embedding. + Data storage servers (expandable multi-terabyte volumes) for archiving client, Pathology Unit, and Imaging Core data. These systems provide rapid access to very large (multi-gigabyte) files or data sets that would otherwise require hours to transfer from other locations in the CNPRC. + Web-based calendar for instrument reservations. + An Olympus BX61 research microscope with 1 color and 2 B/W cameras, Olympus DSU spinning disk confocal system, computer controlled stage and focusing system, Molecular Devices MetaMorph image capture and analysis software, and Intelligent Imaging Innovations SlideBook software for stereology and image data collection. + A second Olympus BX61 microscope with Intelligent Imaging Innovations SlideBook software for stereology technique development by the Core's staff. + A graphics workstation, flatbed scanner, and a high-quality color corrected printer; these are used for publication quality prints, converting film to digital images, image correction, and image archiving. + Document scanners for rapid digitization and OCR conversion of paper records. + A Delta Vision Microscopy System and computer workstation for very high resolution multi-dimensional fluorescence imaging with deconvolution. + A computer workstation for offline analysis of stereology data and large multidimensional image analysis. + A recently updated Visiopharm VIS/CAST Grid Stereology System for quantitative data collection and automated image analysis. + Adobe Photoshop, Adobe Illustrator, and Adobe LightRoom software to support all digital imaging efforts of the Core  all computers are outfitted with spectrophotometrically color balanced monitors. + A fluorescence stereomicroscope outfitted with a high sensitivity color camera coupled to a computer system. + A dual-head brightfield stereomicroscope + An Olympus VS110 virtual microscopy / whole slide scanning system for brightfield, DIC and fluorescence imaging + Cell Biosciences FluorChem-E scanner for gels and blots