COBRE: UNE MED CTR: CORE D: DNA MICROARRAY CORE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 包括人类、小鼠和果蝇在内的几种不同基因组测序的完成，为研究人员提供了巨大的机会， 生物医学进展（Waterston等，2002; Venter等，2001）。DNA微阵列技术是利用序列数据的新技术之一，并且是获得对正常和疾病相关生物过程的关键洞察的强大的高通量手段（霍洛威AJ等人，2002; Shultz和Downward，2001）。虽然这项技术大约在过去十年才发展起来，但它发展迅速，使用微阵列的应用范围也在不断扩大。微阵列实验能够全面评估整体基因表达谱的相对变化，因此可用于阐明指示发育和对环境刺激的反应的转录机制（lyer et al，1999）。它们还可以用于表征疾病状态并将这些 具有预后结果的转录谱（Chung等，2002）。除了设计用于评估转录活性的DNA微阵列之外，还有DNA微阵列， 设计用于表征DNA /蛋白质相互作用以及遗传变异（Pollack和Lyer，2002; Patil N等，2001）。据预测，在未来，DNA微阵列将用于 用于诊断和可能的个性化医疗的临床环境（Petricoin EF等人，2002）。联合国监测委员会DNA微阵列核心设施将支持所概述的COBRE项目，为调查人员在其各自的研究项目中利用DNA微阵列技术提供必要的资源。微阵列技术是一种手段， 可以在生物学途径中获得见解，因此它将帮助初级研究人员获得数据，以显着增加他们在申请方面具有竞争力的可能性。 校外资助

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The completion of the sequencing of several different genomes including the human, mouse, and drosophila genomes, has resulted in tremendous opportunities for researchers to make biomedical advances (Waterston et al, 2002; Venter et al, 2001). DNA microarray technology is one of the new technologies that takes advantage of the sequence data and is a powerful highthroughput means to gain crucial insight into both normal and disease-related biological processes (Holloway AJ et al, 2002; Shultz and Downward, 2001). Although the technology has only been developed in roughly the last ten years, it has advanced rapidly and the list of applications in which microarrays have been used is growing. Microarray experiments are capable of comprehensivelyassessing relative changes in global gene expression profiles and as such can be used to elucidate transcriptional mechanisms dictating development and the response to environmental stimuli (lyer et al, 1999). They can also be used to characterize disease states and associate these transcriptional profiles with prognostic outcome (Chung et al, 2002). In addition to DNA microarrays that are designed to assess transcriptional activity, there are DNA microarrays that are designed to characterize DNA / protein interactions as well as genetic variation (Pollack and lyer, 2002; Patil N et al, 2001). It is also predicted that in the future DNA microarrays will be used in clinical settings for diagnosis and perhaps personalized medicine (Petricoin EF et al, 2002). The UNMC DNA Microarray Core Facility will support the COBRE project as outlined by providing the necessary resources for the investigators to utilize DNA microarray technology in their respective research projects. Microarray technology is a means by which much needed novel insights can be gained in biological pathways, as such it will help the junior investigators obtain data to significantly increase the likelihood that they will be competitive in regard to applying for extramural funding.