MICROSCOPY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 显微镜核心设施为五个单独的项目提供各种技术方面的最先进的专业知识和设备，范围从明场组织和组织化学分析、酶组织化学、免疫组织化学和免疫细胞化学、原位杂交组织化学，到光学显微镜、计算机辅助图像分析和形态测量；从活细胞成像、共聚焦显微镜和3D渲染，到标准的透射电子显微镜、免疫金标记和先进的冷冻技术(冷冻免疫金标记、冷冻替代、高压冷冻、冷冻断裂和深度蚀刻)。该核心为个别研究人员提供培训，调整和校准这些仪器，协助准备标本，并监督实验和维护的日程安排。它以心血管生物学研究计划为基础，靠近其他实验室和科学核心，促进了中心成员之间的高水平互动和合作。该核心在弗洛里亚·卢普博士的指导下运作。作为一名经验丰富的成像专家，Robert Silasi-Mansat博士致力于本提案中描述的活动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Microscopy Core Facility provides state of the art expertise and equipment to the five individual projects on a variety of techniques, ranging from bright-field histological and histochemical analysis, enzyme histochemistry, immunohistochemistry and immunocytochemistry, in situ hybridization histochemistry, to photomicroscopy, computer-assisted image analysis, and morphometry; from live-cell imaging, confocal microscopy and 3D rendering, to standard transmission electron microscopy, immunogold labeling and advanced cryo-technologies (cryoimmunogold labeling, freeze substitution, high pressure freezing, and freeze fracture and deep-etching). The core provides training for individual investigators, align and calibrate these instruments, assist with preparation of specimens, and oversee scheduling of experiments and maintenance. It is based in the Cardiovascular Biology Research Program, in close proximity of the other labs and scientific cores, which promotes a high level of interaction and collaboration between Center members. The core operates under the direction of Dr. Florea Lupu. An experienced imaging specialist, Dr. Robert Silasi-Mansat devotes effort towards the activities described in this proposal.